Light-field Microscopy with Temporal Focusing Multiphoton Illumination for Scanless Volumetric Bioimaging

Overview

Journal Biomed Opt Express

Specialty Radiology

Date 2023 Jan 2

PMID 36589593

Authors

Feng-Chun Hsu

Chun-Yu Lin

Yvonne Yuling Hu

Yeu-Kuang Hwu

Ann-Shyn Chiang

Shean-Jen Chen

Affiliations

Soon will be listed here.

Abstract

A temporal focusing multiphoton illumination (TFMI) method is proposed for achieving selective volume illumination (SVI) (i.e., illuminating only the volume of interest) in light-field microscopy (LFM). The proposed method minimizes the background noise of the LFM images and enhances the contrast, and thus improves the imaging quality. Three-dimensional (3D) volumetric imaging is achieved by reconstructing the LFM images using a phase-space deconvolution algorithm. The experimental results obtained using 100-nm fluorescent beads show that the proposed TFMI-LFM system achieves lateral and axial resolutions of 1.2 µm and 1.1 µm, respectively, at the focal plane. Furthermore, the TFMI-LFM system enables 3D images of the single lobe of the drosophila mushroom body with GFP biomarker (OK-107) to be reconstructed in a one-snapshot record.

Citing Articles

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