Transgenic Tobacco Plant Overexpressing Ginkgo Dihydroflavonol 4-reductase Gene Exhibits Multiple Developmental Defects

Overview

Journal Front Plant Sci

Date 2023 Jan 2

PMID 36589135

Authors

Jun Ni

Ning Zhang

Yang Zhan

Kexin Ding

Peng Qi

Xuejun Wang

Wona Ding

Maojun Xu

Affiliations

Soon will be listed here.

Abstract

Dihydroflavonol Q 4-reductase (DFR), a key enzyme in the flavonoid biosynthetic pathway in plants, significantly influences plant survival. However, the roles of DFR in the regulation of plant development are largely unknown. In the present study, phenotypes of transgenic tobacco plants overexpressing the gene, , were investigated. Transgenic tobacco seedlings exhibited relatively low fresh weights, long primary roots, decreased lateral root numbers, and impaired root gravitropic responses when compared to wild-type tobacco plants. Adult transgenic tobacco plants exhibited a considerably high percentage of wrinkled leaves when compared to the wild-type tobacco plants. In addition to the auxin-related phenotypic changes, transgenic tobacco plants exhibited delayed flowering phenotypes under short-day conditions. Gene expression analysis revealed that the delayed flowering in transgenic tobacco plants was caused by the low expression levels of . Finally, variations in anthocyanin and flavonoid contents in transgenic tobacco plants were evaluated. The results revealed that the levels of most anthocyanins identified in transgenic tobacco leaves increased. Specifically, cyanidin-3,5--diglucoside content increased by 9.8-fold in transgenic tobacco plants when compared to the wild-type tobacco plants. Pelargonidin-3--(coumaryl)-glucoside was only detected in transgenic tobacco plants. Regarding flavonoid compounds, one flavonoid compound (epicatechin gallate) was upregulated, whereas seven flavonoid compounds (Tamarixetin-3--rutinoside; Sexangularetin-3--glucoside-7--rhamnoside; Kaempferol-3--neohesperidoside; Engeletin; 2'-Hydoxy,5-methoxyGenistein--rhamnosyl-glucoside; Diosmetin; Hispidulin) were downregulated in both transgenic tobacco leaves and roots. The results indicate novel and multiple roles of in ginkgo and provide a valuable method to produce a late flowering tobacco variety in tobacco industry.

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References

Ponnu J, Wahl V, Schmid M . Trehalose-6-phosphate: connecting plant metabolism and development. Front Plant Sci. 2012; 2:70. PMC: 3355582. DOI: 10.3389/fpls.2011.00070. View

Lavenus J, Goh T, Roberts I, Guyomarch S, Lucas M, De Smet I . Lateral root development in Arabidopsis: fifty shades of auxin. Trends Plant Sci. 2013; 18(8):450-8. DOI: 10.1016/j.tplants.2013.04.006. View

Harig L, Beinecke F, Oltmanns J, Muth J, Muller O, Ruping B . Proteins from the FLOWERING LOCUS T-like subclade of the PEBP family act antagonistically to regulate floral initiation in tobacco. Plant J. 2012; 72(6):908-21. DOI: 10.1111/j.1365-313X.2012.05125.x. View

Guan C, Wu B, Yu T, Wang Q, Krogan N, Liu X . Spatial Auxin Signaling Controls Leaf Flattening in Arabidopsis. Curr Biol. 2017; 27(19):2940-2950.e4. PMC: 6419953. DOI: 10.1016/j.cub.2017.08.042. View

Kinoshita A, Richter R . Genetic and molecular basis of floral induction in Arabidopsis thaliana. J Exp Bot. 2020; 71(9):2490-2504. PMC: 7210760. DOI: 10.1093/jxb/eraa057. View

Rashotte A, Brady S, Reed R, Ante S, Muday G . Basipetal auxin transport is required for gravitropism in roots of Arabidopsis. Plant Physiol. 2000; 122(2):481-90. PMC: 58885. DOI: 10.1104/pp.122.2.481. View

Santelia D, Henrichs S, Vincenzetti V, Sauer M, Bigler L, Klein M . Flavonoids redirect PIN-mediated polar auxin fluxes during root gravitropic responses. J Biol Chem. 2008; 283(45):31218-26. PMC: 2662185. DOI: 10.1074/jbc.M710122200. View

Wickland D, Hanzawa Y . The FLOWERING LOCUS T/TERMINAL FLOWER 1 Gene Family: Functional Evolution and Molecular Mechanisms. Mol Plant. 2015; 8(7):983-97. DOI: 10.1016/j.molp.2015.01.007. View

Peer W, Murphy A . Flavonoids and auxin transport: modulators or regulators?. Trends Plant Sci. 2008; 12(12):556-63. DOI: 10.1016/j.tplants.2007.10.003. View

10.

Woodward A, Bartel B . Auxin: regulation, action, and interaction. Ann Bot. 2005; 95(5):707-35. PMC: 4246732. DOI: 10.1093/aob/mci083. View

11.

Fraga C, Clowers B, Moore R, Zink E . Signature-discovery approach for sample matching of a nerve-agent precursor using liquid chromatography-mass spectrometry, XCMS, and chemometrics. Anal Chem. 2010; 82(10):4165-73. DOI: 10.1021/ac1003568. View

12.

Liu Y, Yang K, Wei X, Wang X . Revisiting the phosphatidylethanolamine-binding protein (PEBP) gene family reveals cryptic FLOWERING LOCUS T gene homologs in gymnosperms and sheds new light on functional evolution. New Phytol. 2016; 212(3):730-744. DOI: 10.1111/nph.14066. View

13.

Andres F, Coupland G . The genetic basis of flowering responses to seasonal cues. Nat Rev Genet. 2012; 13(9):627-39. DOI: 10.1038/nrg3291. View

14.

Chen W, Gong L, Guo Z, Wang W, Zhang H, Liu X . A novel integrated method for large-scale detection, identification, and quantification of widely targeted metabolites: application in the study of rice metabolomics. Mol Plant. 2013; 6(6):1769-80. DOI: 10.1093/mp/sst080. View

15.

Ni J, Hao J, Jiang Z, Zhan X, Dong L, Yang X . NaCl Induces Flavonoid Biosynthesis through a Putative Novel Pathway in Post-harvest Ginkgo Leaves. Front Plant Sci. 2017; 8:920. PMC: 5466993. DOI: 10.3389/fpls.2017.00920. View

16.

Hua C, Linling L, Shuiyuan C, Fuliang C, Feng X, Honghui Y . Molecular cloning and characterization of three genes encoding dihydroflavonol-4-reductase from Ginkgo biloba in anthocyanin biosynthetic pathway. PLoS One. 2013; 8(8):e72017. PMC: 3753345. DOI: 10.1371/journal.pone.0072017. View

17.

Casimiro I, Marchant A, Bhalerao R, Beeckman T, Dhooge S, Swarup R . Auxin transport promotes Arabidopsis lateral root initiation. Plant Cell. 2001; 13(4):843-52. PMC: 135543. DOI: 10.1105/tpc.13.4.843. View

18.

Wu Y, Wang T, Xin Y, Wang G, Xu L . Overexpression of the ' Gene Enhanced the Epigallocatechin, Gallocatechin, and Catechin Contents in Transgenic . J Agric Food Chem. 2020; 68(4):998-1006. DOI: 10.1021/acs.jafc.9b07008. View

19.

Capovilla G, Schmid M, Pose D . Control of flowering by ambient temperature. J Exp Bot. 2014; 66(1):59-69. DOI: 10.1093/jxb/eru416. View

20.

Singh B, Kaur P, Gopichand , Singh R, Ahuja P . Biology and chemistry of Ginkgo biloba. Fitoterapia. 2008; 79(6):401-18. DOI: 10.1016/j.fitote.2008.05.007. View