[Activity of Curcumin Against Human Cytomegalovirus Infection ]

Overview

Journal Sheng Wu Yi Xue Gong Cheng Xue Za Zhi

Specialty Biomedical Engineering

Date 2022 Dec 27

PMID 36575085

Authors

Xiang Ding

Jirong Yue

Birong Dong

Leng Sean X

Affiliations

Soon will be listed here.

Abstract

This study aimed to investigate the effect of curcumin (Cur) against human cytomegalovirus (HCMV) . Human embryonic lung fibroblasts were cultured . The tetrazolium salt (MTS) method was used to detect the effects of Cur on cell viability. The cells were divided into control group, HCMV group, HCMV + (PFA) group and HCMV + Cur group in this study. The cytopathic effect (CPE) of each group was observed by plaque test, then the copy number of HCMV DNA in each group was detected by quantitative polymerase chain reaction (qPCR), and the expression of HCMV proteins in different sequence was detected by Western blot. The results showed that when the concentration of Cur was not higher than 15 μmol/L, there was no significant change in cell growth and viability in the Cur group compared with the control group (>0.05). After the cells were infected by HCMV for 5 d, the cells began to show CPE, and the number of plaques increased with time. Pretreatment with Cur significantly reduced CPE in a dose-dependent manner. After the cells were infected by HCMV, the DNA copy number and protein expression gradually increased in a time-dependent manner. Pretreatment with Cur significantly inhibited HCMV DNA copies and downregulate HCMV protein expression levels in a concentration-dependent manner, and the difference was statistically significant (<0.05). In conclusion, Cur may exert anti-HCMV activity by inhibiting the replication of HCMV DNA and down-regulating the expression levels of different sequence proteins of HCMV. This study provides a new experimental basis for the development of anti-HCMV infectious drugs.

References

Ho M . The history of cytomegalovirus and its diseases. Med Microbiol Immunol. 2007; 197(2):65-73. DOI: 10.1007/s00430-007-0066-x. View

Mishra A, Kumar R, Tyagi A, Kohaar I, Hedau S, Bharti A . Curcumin modulates cellular AP-1, NF-kB, and HPV16 E6 proteins in oral cancer. Ecancermedicalscience. 2015; 9:525. PMC: 4407748. DOI: 10.3332/ecancer.2015.525. View

Sissons J, Carmichael A . Clinical aspects and management of cytomegalovirus infection. J Infect. 2002; 44(2):78-83. DOI: 10.1053/jinf.2001.0949. View

Ciccocioppo R, Racca F, Scudeller L, Piralla A, Formagnana P, Pozzi L . Differential cellular localization of Epstein-Barr virus and human cytomegalovirus in the colonic mucosa of patients with active or quiescent inflammatory bowel disease. Immunol Res. 2015; 64(1):191-203. DOI: 10.1007/s12026-015-8737-y. View

Wang Z, Peng X, Li M, Jin F, Zhang B, Wang H . Is human cytomegalovirus infection associated with essential hypertension? A meta-analysis of 11,878 participants. J Med Virol. 2015; 88(5):852-8. DOI: 10.1002/jmv.24391. View

Jergovic M, Contreras N, Nikolich-Zugich J . Impact of CMV upon immune aging: facts and fiction. Med Microbiol Immunol. 2019; 208(3-4):263-269. PMC: 6635032. DOI: 10.1007/s00430-019-00605-w. View

Winston D, Ho W, Champlin R . Cytomegalovirus infections after allogeneic bone marrow transplantation. Rev Infect Dis. 1990; 12 Suppl 7:S776-92. DOI: 10.1093/clinids/12.supplement_7.s776. View

Zhu W, Liu S . The role of human cytomegalovirus in atherosclerosis: a systematic review. Acta Biochim Biophys Sin (Shanghai). 2020; 52(4):339-353. DOI: 10.1093/abbs/gmaa005. View

Han S, Xu J, Guo X, Huang M . Curcumin ameliorates severe influenza pneumonia via attenuating lung injury and regulating macrophage cytokines production. Clin Exp Pharmacol Physiol. 2017; 45(1):84-93. DOI: 10.1111/1440-1681.12848. View

10.

Rubin R, Oliver D, ROTA T, Hamilton J, Betts R, Pass R . Multicenter seroepidemiologic study of the impact of cytomegalovirus infection on renal transplantation. Transplantation. 1985; 40(3):243-9. DOI: 10.1097/00007890-198509000-00004. View

11.

Jacobson M, Mills J . Serious cytomegalovirus disease in the acquired immunodeficiency syndrome (AIDS). Clinical findings, diagnosis, and treatment. Ann Intern Med. 1988; 108(4):585-94. DOI: 10.7326/0003-4819-108-4-585. View

12.

Aiello A, Haan M, Blythe L, Moore K, Gonzalez J, Jagust W . The influence of latent viral infection on rate of cognitive decline over 4 years. J Am Geriatr Soc. 2006; 54(7):1046-54. DOI: 10.1111/j.1532-5415.2006.00796.x. View

13.

Aiello A, Haan M, Pierce C, Simanek A, Liang J . Persistent infection, inflammation, and functional impairment in older Latinos. J Gerontol A Biol Sci Med Sci. 2008; 63(6):610-8. PMC: 3178457. DOI: 10.1093/gerona/63.6.610. View

14.

Pari G . Nuts and bolts of human cytomegalovirus lytic DNA replication. Curr Top Microbiol Immunol. 2008; 325:153-66. DOI: 10.1007/978-3-540-77349-8_9. View

15.

Lv Y, Lei N, Wang D, An Z, Li G, Han F . Protective effect of curcumin against cytomegalovirus infection in Balb/c mice. Environ Toxicol Pharmacol. 2014; 37(3):1140-7. DOI: 10.1016/j.etap.2014.04.017. View

16.

Staras S, Dollard S, Radford K, Flanders W, Pass R, Cannon M . Seroprevalence of cytomegalovirus infection in the United States, 1988-1994. Clin Infect Dis. 2006; 43(9):1143-51. DOI: 10.1086/508173. View

17.

Griffiths P, Baraniak I, Reeves M . The pathogenesis of human cytomegalovirus. J Pathol. 2014; 235(2):288-97. DOI: 10.1002/path.4437. View

18.

Roberts B, Cech I . Association of type 2 diabetes mellitus and seroprevalence for cytomegalovirus. South Med J. 2005; 98(7):686-92. DOI: 10.1097/01.SMJ.0000163310.12516.2D. View

19.

Cannon M, Schmid D, Hyde T . Review of cytomegalovirus seroprevalence and demographic characteristics associated with infection. Rev Med Virol. 2010; 20(4):202-13. DOI: 10.1002/rmv.655. View

20.

Lv Y, An Z, Chen H, Wang Z, Liu L . Mechanism of curcumin resistance to human cytomegalovirus in HELF cells. BMC Complement Altern Med. 2014; 14:284. PMC: 4132927. DOI: 10.1186/1472-6882-14-284. View