Technologies for Viable Circulating Tumor Cell Isolation

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2022 Dec 23

PMID 36555625

Authors

Maria S Tretyakova

Maxim E Menyailo

Anastasia A Schegoleva

Ustinia A Bokova

Irina V Larionova

Evgeny V Denisov

Affiliations

Soon will be listed here.

Abstract

The spread of tumor cells throughout the body by traveling through the bloodstream is a critical step in metastasis, which continues to be the main cause of cancer-related death. The detection and analysis of circulating tumor cells (CTCs) is important for understanding the biology of metastasis and the development of antimetastatic therapy. However, the isolation of CTCs is challenging due to their high heterogeneity and low representation in the bloodstream. Different isolation methods have been suggested, but most of them lead to CTC damage. However, viable CTCs are an effective source for developing preclinical models to perform drug screening and model the metastatic cascade. In this review, we summarize the available literature on methods for isolating viable CTCs based on different properties of cells. Particular attention is paid to the importance of in vitro and in vivo models obtained from CTCs. Finally, we emphasize the current limitations in CTC isolation and suggest potential solutions to overcome them.

Citing Articles

Multifaceted Approaches in Epithelial Cell Adhesion Molecule-Mediated Circulating Tumor Cell Isolation.

Szerenyi D, Jarvas G, Guttman A Molecules. 2025; 30(5).

PMID: 40076201 PMC: 11901967. DOI: 10.3390/molecules30050976.

Combining rVAR2 and Anti-EpCAM to Increase the Capture Efficiency of Non-Small-Cell Lung Cancer Cell Lines in the Flow Enrichment Target Capture Halbach (FETCH) Magnetic Separation System.

He S, Liu P, Wu Y, Agerbaek M, Salanti A, Terstappen L Int J Mol Sci. 2024; 25(18).

PMID: 39337304 PMC: 11432156. DOI: 10.3390/ijms25189816.

Deformation under flow and morphological recovery of cancer cells.

Gasser E, Su E, Vaidziulyte K, Abbade N, Cognart H, Manneville J Lab Chip. 2024; 24(16):3930-3944.

PMID: 38993177 PMC: 11302772. DOI: 10.1039/d4lc00246f.

Circulating tumor cells shielded with extracellular vesicle-derived CD45 evade T cell attack to enable metastasis.

Yang C, Wang X, To K, Cui C, Luo M, Wu S Signal Transduct Target Ther. 2024; 9(1):84.

PMID: 38575583 PMC: 10995208. DOI: 10.1038/s41392-024-01789-1.

Advancements in Circulating Tumor Cell Research: Bridging Biology and Clinical Applications.

Salu P, Reindl K Cancers (Basel). 2024; 16(6).

PMID: 38539545 PMC: 10969710. DOI: 10.3390/cancers16061213.

References

Galletti G, Sung M, Vahdat L, Shah M, Santana S, Altavilla G . Isolation of breast cancer and gastric cancer circulating tumor cells by use of an anti HER2-based microfluidic device. Lab Chip. 2013; 14(1):147-56. PMC: 3921117. DOI: 10.1039/c3lc51039e. View

Bork U, Rahbari N, Scholch S, Reissfelder C, Kahlert C, Buchler M . Circulating tumour cells and outcome in non-metastatic colorectal cancer: a prospective study. Br J Cancer. 2015; 112(8):1306-13. PMC: 4402459. DOI: 10.1038/bjc.2015.88. View

Aceto N, Bardia A, Miyamoto D, Donaldson M, Wittner B, Spencer J . Circulating tumor cell clusters are oligoclonal precursors of breast cancer metastasis. Cell. 2014; 158(5):1110-1122. PMC: 4149753. DOI: 10.1016/j.cell.2014.07.013. View

de Bono J, Scher H, Montgomery R, Parker C, Miller M, Tissing H . Circulating tumor cells predict survival benefit from treatment in metastatic castration-resistant prostate cancer. Clin Cancer Res. 2008; 14(19):6302-9. DOI: 10.1158/1078-0432.CCR-08-0872. View

Waheed W, Sharaf O, Alazzam A, Abu-Nada E . Dielectrophoresis-field flow fractionation for separation of particles: A critical review. J Chromatogr A. 2021; 1637:461799. DOI: 10.1016/j.chroma.2020.461799. View

Zhang H, Yuan F, Qi Y, Liu B, Chen Q . Circulating Tumor Cells for Glioma. Front Oncol. 2021; 11:607150. PMC: 7987781. DOI: 10.3389/fonc.2021.607150. View

Soler A, Cayrefourcq L, Mazard T, Babayan A, Lamy P, Assou S . Autologous cell lines from circulating colon cancer cells captured from sequential liquid biopsies as model to study therapy-driven tumor changes. Sci Rep. 2018; 8(1):15931. PMC: 6206091. DOI: 10.1038/s41598-018-34365-z. View

Baek D, Kim G, Song G, Han I, Park E, Kim H . Clinical Potential of Circulating Tumor Cells in Colorectal Cancer: A Prospective Study. Clin Transl Gastroenterol. 2019; 10(7):e00055. PMC: 6708664. DOI: 10.14309/ctg.0000000000000055. View

Pereira-Veiga T, Abreu M, Robledo D, Matias-Guiu X, Santacana M, Sanchez L . CTCs-derived xenograft development in a triple negative breast cancer case. Int J Cancer. 2018; 144(9):2254-2265. DOI: 10.1002/ijc.32001. View

10.

Lampignano R, Yang L, Neumann M, Franken A, Fehm T, Niederacher D . A Novel Workflow to Enrich and Isolate Patient-Matched EpCAM and EpCAM CTCs Enables the Comparative Characterization of the PIK3CA Status in Metastatic Breast Cancer. Int J Mol Sci. 2017; 18(9). PMC: 5618534. DOI: 10.3390/ijms18091885. View

11.

Jolly M, Murphy R, Bhatia S, Whitfield H, Redfern A, Davis M . Measuring and Modelling the Epithelial- Mesenchymal Hybrid State in Cancer: Clinical Implications. Cells Tissues Organs. 2021; 211(2):110-133. DOI: 10.1159/000515289. View

12.

Sharma S, Zhuang R, Long M, Pavlovic M, Kang Y, Ilyas A . Circulating tumor cell isolation, culture, and downstream molecular analysis. Biotechnol Adv. 2018; 36(4):1063-1078. PMC: 5971144. DOI: 10.1016/j.biotechadv.2018.03.007. View

13.

Carmona-Ule N, Gonzalez-Conde M, Abuin C, Cueva J, Palacios P, Lopez-Lopez R . Short-Term Ex Vivo Culture of CTCs from Advance Breast Cancer Patients: Clinical Implications. Cancers (Basel). 2021; 13(11). PMC: 8198105. DOI: 10.3390/cancers13112668. View

14.

Cayrefourcq L, Thomas F, Mazard T, Assenat E, Assou S, Alix-Panabieres C . Selective treatment pressure in colon cancer drives the molecular profile of resistant circulating tumor cell clones. Mol Cancer. 2021; 20(1):30. PMC: 7869222. DOI: 10.1186/s12943-021-01326-6. View

15.

Song P, Ye D, Zuo X, Li J, Wang J, Liu H . DNA Hydrogel with Aptamer-Toehold-Based Recognition, Cloaking, and Decloaking of Circulating Tumor Cells for Live Cell Analysis. Nano Lett. 2017; 17(9):5193-5198. DOI: 10.1021/acs.nanolett.7b01006. View

16.

Savelieva O, Tashireva L, Kaigorodova E, Buzenkova A, Mukhamedzhanov R, Grigoryeva E . Heterogeneity of Stemlike Circulating Tumor Cells in Invasive Breast Cancer. Int J Mol Sci. 2020; 21(8). PMC: 7216207. DOI: 10.3390/ijms21082780. View

17.

Su P, Lai W, Liu L, Zeng Y, Xu H, Lan Q . Mesenchymal and Phosphatase of Regenerating Liver-3 Status in Circulating Tumor Cells May Serve as a Crucial Prognostic Marker for Assessing Relapse or Metastasis in Postoperative Patients With Colorectal Cancer. Clin Transl Gastroenterol. 2021; 11(12):e00265. PMC: 7743843. DOI: 10.14309/ctg.0000000000000265. View

18.

Gabriel M, Rodriguez Calleja L, Chalopin A, Ory B, Heymann D . Circulating Tumor Cells: A Review of Non-EpCAM-Based Approaches for Cell Enrichment and Isolation. Clin Chem. 2016; 62(4):571-81. DOI: 10.1373/clinchem.2015.249706. View

19.

Brungs D, Lynch D, Luk A, Minaei E, Ranson M, Aghmesheh M . Cryopreservation for delayed circulating tumor cell isolation is a valid strategy for prognostic association of circulating tumor cells in gastroesophageal cancer. World J Gastroenterol. 2018; 24(7):810-818. PMC: 5807939. DOI: 10.3748/wjg.v24.i7.810. View

20.

Mikolajczyk S, Millar L, Tsinberg P, Coutts S, Zomorrodi M, Pham T . Detection of EpCAM-Negative and Cytokeratin-Negative Circulating Tumor Cells in Peripheral Blood. J Oncol. 2011; 2011:252361. PMC: 3090615. DOI: 10.1155/2011/252361. View