Does Photobiomodulation Affects CK10 and CK14 in Oral Mucositis Radioinduced Repair?

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2022 Dec 23

PMID 36555260

Authors

Ariane Venzon Naia Sardo

Maira Franco Andrade

Anaeliza Figueiredo

Flavia Cristina Perillo Rosin

Luciana Correa

Denise Maria Zezell

Affiliations

Soon will be listed here.

Abstract

The mechanisms of action of photobiomodulation (PBM) in oral mucositis (OM) are not completely elucidated. To enlighten the role of PBM in the evolution of epithelial maturity in OM ulcers, the present study evaluated the effect of PBM with red (λ) wavelength of 660 nanometers (nm) and infrared of 780 nm in radio-induced OM wounds on the tongue of rats, eight and twenty days after irradiation with single dose of 20 Gy. The percentage area corresponding to positive staining for cytokeratin 10 (CK10) and 14 (CK14) proteins was evaluated in the epithelial area of the lesions, using an immunohistochemical technique (IHC), 8 and 20 days after the induction of lesions, and compared with an untreated control group. CK10 was significantly more expressed in the group treated with 660 nm PBM. CK14 did not show quantitative differences between the groups evaluated. However, whereas in the groups treated with PBM, CK14 was already restricted to the basal layer of the epithelium, as expected in healthy epithelia, in control group it was also expressed in upper layers of the epithelium. In this work, PBM was able to improve epithelial maturity of the repaired OM wound, especially in the 660 nm group.

References

Cini N, Gruber S, Arican Alicikus Z, Dorr W . Modulation of radiation-induced oral mucositis (mouse) by dermatan sulfate: effects on differentiation processes. Strahlenther Onkol. 2019; 196(1):85-94. PMC: 6957576. DOI: 10.1007/s00066-019-01532-8. View

Sawaf M, Ouhayoun J, FOREST N . Cytokeratin profiles in oral epithelial: a review and a new classification. J Biol Buccale. 1991; 19(3):187-98. View

Epstein J, Thariat J, Bensadoun R, Barasch A, Murphy B, Kolnick L . Oral complications of cancer and cancer therapy: from cancer treatment to survivorship. CA Cancer J Clin. 2012; 62(6):400-22. DOI: 10.3322/caac.21157. View

Dale B, Holbrook K, Kimball J, Hoff M, Sun T . Expression of epidermal keratins and filaggrin during human fetal skin development. J Cell Biol. 1985; 101(4):1257-69. PMC: 2113922. DOI: 10.1083/jcb.101.4.1257. View

Aragon-Sanchez J, Quintana-Marrero Y, Aragon-Hernandez C, Hernandez-Herero M . ImageJ: A Free, Easy, and Reliable Method to Measure Leg Ulcers Using Digital Pictures. Int J Low Extrem Wounds. 2017; 16(4):269-273. DOI: 10.1177/1534734617744951. View

Werner S, Krieg T, Smola H . Keratinocyte-fibroblast interactions in wound healing. J Invest Dermatol. 2007; 127(5):998-1008. DOI: 10.1038/sj.jid.5700786. View

Sonis S . Mucositis: The impact, biology and therapeutic opportunities of oral mucositis. Oral Oncol. 2009; 45(12):1015-20. DOI: 10.1016/j.oraloncology.2009.08.006. View

Kim J, Jung M, Kim J, Kim H, Jung J, Hahm J . Alpha lipoic acid attenuates radiation-induced oral mucositis in rats. Oncotarget. 2017; 8(42):72739-72747. PMC: 5641165. DOI: 10.18632/oncotarget.20286. View

Peng H, Chen B, Chen L, Chen Y, Liu X, Tang L . A network meta-analysis in comparing prophylactic treatments of radiotherapy-induced oral mucositis for patients with head and neck cancers receiving radiotherapy. Oral Oncol. 2017; 75:89-94. DOI: 10.1016/j.oraloncology.2017.11.001. View

10.

Coulombe P, Omary M . 'Hard' and 'soft' principles defining the structure, function and regulation of keratin intermediate filaments. Curr Opin Cell Biol. 2002; 14(1):110-22. DOI: 10.1016/s0955-0674(01)00301-5. View

11.

Moll R, Franke W, Schiller D, Geiger B, Krepler R . The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell. 1982; 31(1):11-24. DOI: 10.1016/0092-8674(82)90400-7. View

12.

El Bousaadani A, Eljahd L, Abada R, Rouadi S, Roubal M, Mahtar M . [Prevention and treatment of mucositis in children with oral cancers: Practical recommendations]. Cancer Radiother. 2016; 20(3):226-30. DOI: 10.1016/j.canrad.2015.11.006. View

13.

Eduardo F, Bezinelli L, de Carvalho D, Marques da Graca Lopes R, Fernandes J, Brumatti M . Oral mucositis in pediatric patients undergoing hematopoietic stem cell transplantation: clinical outcomes in a context of specialized oral care using low-level laser therapy. Pediatr Transplant. 2015; 19(3):316-25. DOI: 10.1111/petr.12440. View

14.

Rusu D, Calenic B, Greabu M, Kralev A, Boariu M, Bojin F . Evaluation of oral keratinocyte progenitor and T-lymphocite cells response during early healing after augmentation of keratinized gingiva with a 3D collagen matrix - a pilot study. BMC Oral Health. 2016; 17(1):9. PMC: 4948093. DOI: 10.1186/s12903-016-0240-x. View

15.

Zadik Y, Arany P, Fregnani E, Bossi P, Spindola Antunes H, Bensadoun R . Systematic review of photobiomodulation for the management of oral mucositis in cancer patients and clinical practice guidelines. Support Care Cancer. 2019; 27(10):3969-3983. DOI: 10.1007/s00520-019-04890-2. View

16.

Sonis S . New thoughts on the initiation of mucositis. Oral Dis. 2010; 16(7):597-600. DOI: 10.1111/j.1601-0825.2010.01681.x. View

17.

de Castro J, da Silva Pereira F, Chen L, Arana-Chavez V, Yague Ballester R, DiPietro L . Improvement of full-thickness rat skin wounds by photobiomodulation therapy (PBMT): A dosimetric study. J Photochem Photobiol B. 2020; 206:111850. DOI: 10.1016/j.jphotobiol.2020.111850. View

18.

Lalla R, Brennan M, Gordon S, Sonis S, Rosenthal D, Keefe D . Oral Mucositis Due to High-Dose Chemotherapy and/or Head and Neck Radiation Therapy. J Natl Cancer Inst Monogr. 2019; 2019(53). DOI: 10.1093/jncimonographs/lgz011. View

19.

Hong C, Gueiros L, Fulton J, Cheng K, Kandwal A, Galiti D . Systematic review of basic oral care for the management of oral mucositis in cancer patients and clinical practice guidelines. Support Care Cancer. 2019; 27(10):3949-3967. DOI: 10.1007/s00520-019-04848-4. View

20.

Sperandio F, Simoes A, Correa L, Aranha A, Giudice F, Hamblin M . Low-level laser irradiation promotes the proliferation and maturation of keratinocytes during epithelial wound repair. J Biophotonics. 2014; 8(10):795-803. PMC: 4583360. DOI: 10.1002/jbio.201400064. View