Ethanol Extraction Promotes Dextran Sulphate Sodium Induced Colitis Recovery and Modulation in Microbiota

Overview

Journal Foods

Specialty Biotechnology

Date 2022 Dec 23

PMID 36553765

Authors

Miaoyu Li

Leilei Yu

Qixiao Zhai

Bingshu Liu

Jianxin Zhao

Wei Chen

Fengwei Tian

Affiliations

Soon will be listed here.

Abstract

Popular edible mushrooms and can improve physical health as a prebiotic and positively alter intestinal microbiota. Our research investigated the prebiotic effects of and on colon inflammation through water extraction polysaccharides (GLP), water extraction polysaccharides (GIP), ethanol extraction (GLE), and ethanol extraction (GIE) administered in mice after 7 days of dextran sulphate sodium (DSS) administration. Among the extracts, GLE showed reduced mortality rates, prevention of weight loss, mitigated colon length shortening, and decreased disease activity indices and histological scores. COX-2, MPO, and iNOS activities and the inflammatory cytokines' expressions were determined to demonstrate the inhibition inflammation by GLE. Meanwhile, GLE upregulated the levels of MUC2, ZO-1, claudin-3, and occluding to protect the intestinal barrier. Furthermore, GLE modulated the composition of gut microbiota disturbed by DSS, as it decreased the abundance of , , and _, and increased and Through cell experiment, GLE had a positive influence on adherens junction, tight junction, and TRAF6/MyD88/NF-κB signaling pathways. In conclusion, GLE supplementation promotes DSS-induced colitis recovery by regulating inflammatory cytokines, preserving the intestinal mucosal barrier, positively modulating microbiota changes, and positively influences immune response in TRAF6/MyD88/NF-κB signaling pathways.

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