Plasma Metabolomic Profiling Reveals Preliminary Biomarkers of Pork Quality Based on PH Value

Overview

Journal Foods

Specialty Biotechnology

Date 2022 Dec 23

PMID 36553747

Authors

Linyuan Shen

Jianfeng Ma

Haodi Zhou

Lei Chen

Jie Tang

Kaige Zhang

Ye Zhao

Lili Niu

Shunhua Zhang

Anan Jiang

Jinyong Wang

Zongyi Guo

Xuewei Li

Yiwu Chen

Mailin Gan

Li Zhu

Affiliations

Soon will be listed here.

Abstract

This study aimed to identify biomarkers for pork quality evaluation. Firstly, the correlation between indicators of pork quality evaluation was investigated. The pH of pork meat at 45 min post slaughter showed a significant negative correlation with meat color indicators (: -0.4868--0.3040). Subsequently, porcine plasma samples were further divided into low pH (pH = 6.16 ± 0.22) or high pH (pH = 6.75 ± 0.08) groups. Plasma metabolites in both sample groups were investigated using untargeted metabolomics. In total, 90 metabolites were recognized as differential metabolites using partial least squares discriminant analysis. Pathway enrichment analysis indicated these differential metabolites were enriched in amino acid metabolism and energy metabolism. Correlation analysis revealed that creatinine, L-carnitine, D-sphingosine, citraconic acid, and other metabolites may constitute novel plasma biomarkers with the pH value of pork meat. The current study provides important insights into plasma biomarkers for predicting pork quality based on pH value.

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References

Song B, Zheng C, Zheng J, Zhang S, Zhong Y, Guo Q . Comparisons of carcass traits, meat quality, and serum metabolome between Shaziling and Yorkshire pigs. Anim Nutr. 2022; 8(1):125-134. PMC: 8669263. DOI: 10.1016/j.aninu.2021.06.011. View

Ramanathan R, Mancini R, Dady G . Effects of pyruvate, succinate, and lactate enhancement on beef longissimus raw color. Meat Sci. 2011; 88(3):424-8. DOI: 10.1016/j.meatsci.2011.01.021. View

Welzenbach J, Neuhoff C, Looft C, Schellander K, Tholen E, Grosse-Brinkhaus C . Different Statistical Approaches to Investigate Porcine Muscle Metabolome Profiles to Highlight New Biomarkers for Pork Quality Assessment. PLoS One. 2016; 11(2):e0149758. PMC: 4769069. DOI: 10.1371/journal.pone.0149758. View

Honikel K . Reference methods for the assessment of physical characteristics of meat. Meat Sci. 2011; 49(4):447-57. DOI: 10.1016/s0309-1740(98)00034-5. View

Otto G, Roehe R, Looft H, Thoelking L, Kalm E . Comparison of different methods for determination of drip loss and their relationships to meat quality and carcass characteristics in pigs. Meat Sci. 2011; 68(3):401-9. DOI: 10.1016/j.meatsci.2004.04.007. View

Welzenbach J, Neuhoff C, Heidt H, Cinar M, Looft C, Schellander K . Integrative Analysis of Metabolomic, Proteomic and Genomic Data to Reveal Functional Pathways and Candidate Genes for Drip Loss in Pigs. Int J Mol Sci. 2016; 17(9). PMC: 5037705. DOI: 10.3390/ijms17091426. View

Fang L, Jin Y, Do S, Hong J, Kim B, Han T . Effects of dietary energy and crude protein levels on growth performance, blood profiles, and carcass traits in growing-finishing pigs. J Anim Sci Technol. 2019; 61(4):204-215. PMC: 6686147. DOI: 10.5187/jast.2019.61.4.204. View

Zhang Y, Zhang J, Gong H, Cui L, Zhang W, Ma J . Genetic correlation of fatty acid composition with growth, carcass, fat deposition and meat quality traits based on GWAS data in six pig populations. Meat Sci. 2018; 150:47-55. DOI: 10.1016/j.meatsci.2018.12.008. View

Hayat M, Kaka U, Sazili A . Assessment of Physicochemical Characteristics and Microbiological Quality in Broiler Chicken Breast Muscle () Subjected to Different Temperatures and Lengths of Cold Transportation. Foods. 2021; 10(4). PMC: 8072825. DOI: 10.3390/foods10040874. View

10.

Clemmons B, Powers J, Campagna S, Seay T, Embree M, Myer P . Rumen fluid metabolomics of beef steers differing in feed efficiency. Metabolomics. 2020; 16(2):23. DOI: 10.1007/s11306-020-1643-x. View

11.

Goldansaz S, Guo A, Sajed T, Steele M, Plastow G, Wishart D . Livestock metabolomics and the livestock metabolome: A systematic review. PLoS One. 2017; 12(5):e0177675. PMC: 5439675. DOI: 10.1371/journal.pone.0177675. View

12.

Scheffler T, Gerrard D . Mechanisms controlling pork quality development: The biochemistry controlling postmortem energy metabolism. Meat Sci. 2011; 77(1):7-16. DOI: 10.1016/j.meatsci.2007.04.024. View

13.

Yang Y, Yang J, Ma J, Yu Q, Han L . iTRAQ-mediated analysis of the relationship between proteomic changes and yak longissimus lumborum tenderness over the course of postmortem storage. Sci Rep. 2021; 11(1):10450. PMC: 8128915. DOI: 10.1038/s41598-021-90012-0. View

14.

Koeth R, Wang Z, Levison B, Buffa J, Org E, Sheehy B . Intestinal microbiota metabolism of L-carnitine, a nutrient in red meat, promotes atherosclerosis. Nat Med. 2013; 19(5):576-85. PMC: 3650111. DOI: 10.1038/nm.3145. View

15.

Gan M, Shen L, Chen L, Jiang D, Jiang Y, Li Q . Meat Quality, Amino Acid, and Fatty Acid Composition of Liangshan Pigs at Different Weights. Animals (Basel). 2020; 10(5). PMC: 7278381. DOI: 10.3390/ani10050822. View

16.

Bararpour N, Gilardi F, Carmeli C, Sidibe J, Ivanisevic J, Caputo T . DBnorm as an R package for the comparison and selection of appropriate statistical methods for batch effect correction in metabolomic studies. Sci Rep. 2021; 11(1):5657. PMC: 7952378. DOI: 10.1038/s41598-021-84824-3. View

17.

Sidwick K, Johnson A, Adam C, Pereira L, Thompson D . Use of Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry and Metabonomic Profiling To Differentiate between Normally Slaughtered and Dead on Arrival Poultry Meat. Anal Chem. 2017; 89(22):12131-12136. DOI: 10.1021/acs.analchem.7b02749. View

18.

Wang C, Matarneh S, Gerrard D, Tan J . Contributions of energy pathways to ATP production and pH variations in postmortem muscles. Meat Sci. 2022; 189:108828. DOI: 10.1016/j.meatsci.2022.108828. View

19.

Tan C, Selamat J, Jambari N, Sukor R, Murugesu S, Khatib A . Muscle and Serum Metabolomics for Different Chicken Breeds under Commercial Conditions by GC-MS. Foods. 2021; 10(9). PMC: 8467607. DOI: 10.3390/foods10092174. View

20.

Saeed O, Sazili A, Akit H, Ebrahimi M, Alimon A, Samsudin A . Effects of corn supplementation on meat quality and fatty acid composition of Dorper lambs fed PKC-Urea treated rice straw. BMC Vet Res. 2019; 15(1):233. PMC: 6615220. DOI: 10.1186/s12917-019-1976-8. View