Molecular Characterization, Purification, and Mode of Action of KAE01 from Lactic Acid Bacteria and Its In Silico Analysis Against MDR/ESBL

Overview

Journal Genes (Basel)

Publisher MDPI

Date 2022 Dec 23

PMID 36553599

Authors

Asma Bashir

Kashif Ali

Khair Bux

Neha Farid

Mitra Khaireabadi

Khwaja Ali Hassan

Abrar Hussain

Kiran Fatima

Shahab Mehmood

Syed Ali Haider

Ralf Herwig

Affiliations

Soon will be listed here.

Abstract

Bacteriocins are gaining immense importance in therapeutics since they show significant antibacterial potential. This study reports the bacteriocin KAE01 from along with its characterization, molecular modeling, and antibacterial potency, by targeting the matrix protein of . The bacteriocin was purified by using ammonium sulfate precipitation and fast protein liquid chromatography (FPLC), and its molecular weight was estimated as 55 kDa by means of SDS-PAGE. The bacteriocin was found to show stability in a wide range of pH values (2.0-10.0) and temperatures (100 °C for 1 h and 121 °C for 15 min). Antimicrobial screening of the purified peptide against different strains of showed its significant antibacterial potential. Scanning electron microscopy of bacteriocin-induced bacterial cultures revealed significant changes in the cellular morphology of the pathogens. In silico molecular modeling of KAE01, followed by molecular docking of the matrix protein (qSA) of and KAE01, supported the antibacterial potency and SEM findings of this study.

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