Individual Differences and Knockout in Zebrafish Reveal Similar Cognitive Effects of BDNF Between Teleosts and Mammals

Overview

Journal Proc Biol Sci

Specialty Biology

Date 2022 Dec 21

PMID 36541170

Authors

Tyrone Lucon-Xiccato

Giulia Montalbano

Elia Gatto

Elena Frigato

Salvatore DAniello

Cristiano Bertolucci

Affiliations

Soon will be listed here.

Abstract

The remarkable similarities in cognitive performance between teleosts and mammals suggest that the underlying cognitive mechanisms might also be similar in these two groups. We tested this hypothesis by assessing the effects of the brain-derived neurotrophic factor (BDNF), which is critical for mammalian cognitive functioning, on fish's cognitive abilities. We found that individual differences in zebrafish's learning abilities were positively correlated with expression. Moreover, a CRISPR/Cas9 mutant zebrafish line that lacks the BDNF gene () showed remarkable learning deficits. Half of the mutants failed a colour discrimination task, whereas the remaining mutants learned the task slowly, taking three times longer than control zebrafish. The mutants also took twice as long to acquire a T-maze task compared to control zebrafish and showed difficulties exerting inhibitory control. An analysis of habituation learning revealed that cognitive impairment in mutants emerges early during development, but could be rescued with a synthetic BDNF agonist. Overall, our study indicates that BDNF has a similar activational effect on cognitive performance in zebrafish and in mammals, supporting the idea that its function is conserved in vertebrates.

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