» Articles » PMID: 36499098

New Insights into Dose-Dependent Effects of Curcumin on ARPE-19 Cells

Overview
Journal Int J Mol Sci
Publisher MDPI
Date 2022 Dec 11
PMID 36499098
Authors
Affiliations
Soon will be listed here.
Abstract

Opposing dose-dependent effects of curcumin (Cur) have been documented in Retinal Pigment Epithelium (RPE); therefore, to shed the light on the mechanisms of action is crucial for ophthalmic applications. On this basis we explored new insights about the dose-dependent mechanisms triggered by Cur in human retinal pigment epithelial cells (ARPE-19). Three concentrations (0.01 mM; 0.05 mM; 0.1 mM) of Cur were tested, followed by morphological, molecular, and functional analysis of the cells. Cur 0.01 mM promotes a significant increase in cell proliferation, not affecting cell cycle progression and apoptosis; by contrast, Cur 0.05 mM and 0.1 mM block cellular proliferation and trigger S-phase cell cycle arrest without inducing apoptosis. The observation of morphological changes in Cur 0.05 mM and 0.1 mM were not associated with neuronal differentiation, as observed by the quantification of Neurofilament-200 and by the analysis of voltage-dependent currents by patch clamp. Evaluation of autophagic markers LC3BII and p62 revealed significant modulations, suggesting an important activation of autophagy in ARPE-19 cells treated with Cur 0.05 mM and Cur 0.1 mM; conversely, Cur 0.01 mM did not affect autophagy. Altogether, our findings show new dose-dependent mechanisms of action of Cur that suggest a wide therapeutic application in ocular diseases with different pathogenesis (i.e., proliferative vitreoretinopathy or Age-Related Macular Degeneration).

Citing Articles

Acid-Unlocked Two-Layer Ca-Loaded Nanoplatform to Interfere With Mitochondria for Synergistic Tumor Therapy.

Zheng Y, Williams G, Hu R, Tong S, Xu J, Wang T Int J Nanomedicine. 2025; 20:1899-1920.

PMID: 39963419 PMC: 11830942. DOI: 10.2147/IJN.S503248.


Curcumin in Ophthalmology: Mechanisms, Challenges, and Emerging Opportunities.

Ribeiro A, Oliveira D, Cabral-Marques H Molecules. 2025; 30(3).

PMID: 39942561 PMC: 11820683. DOI: 10.3390/molecules30030457.


Machine Learning Elucidates Design Features of Plasmid Deoxyribonucleic Acid Lipid Nanoparticles for Cell Type-Preferential Transfection.

Cheng L, Zhu Y, Ma J, Aggarwal A, Toh W, Shin C ACS Nano. 2024; 18(42):28735-28747.

PMID: 39375194 PMC: 11512640. DOI: 10.1021/acsnano.4c07615.


Effectiveness and safety of adjuvant treatment of tislelizumab with or without chemotherapy in patients with high-risk upper tract urothelial carcinoma: a retrospective, real-world study.

Quan P, Zhang L, Yang B, Hou H, Wu N, Fan X Clin Transl Oncol. 2024; .

PMID: 39172333 DOI: 10.1007/s12094-024-03659-2.


Small Extracellular Vesicles and Oxidative Pathophysiological Mechanisms in Retinal Degenerative Diseases.

Romero F, Diaz-Llopis M, Romero-Gomez M, Miranda M, Romero-Wenz R, Sancho-Pelluz J Int J Mol Sci. 2024; 25(3).

PMID: 38338894 PMC: 10855665. DOI: 10.3390/ijms25031618.

References
1.
Chang Y, Chang W, Hung K, Yang D, Cheng Y, Liao Y . The generation of induced pluripotent stem cells for macular degeneration as a drug screening platform: identification of curcumin as a protective agent for retinal pigment epithelial cells against oxidative stress. Front Aging Neurosci. 2014; 6:191. PMC: 4117985. DOI: 10.3389/fnagi.2014.00191. View

2.
Platania C, Fidilio A, Lazzara F, Piazza C, Geraci F, Giurdanella G . Retinal Protection and Distribution of Curcumin and . Front Pharmacol. 2018; 9:670. PMC: 6036289. DOI: 10.3389/fphar.2018.00670. View

3.
Patel S, Acharya A, Ray R, Agrawal R, Raghuwanshi R, Jain P . Cellular and molecular mechanisms of curcumin in prevention and treatment of disease. Crit Rev Food Sci Nutr. 2019; 60(6):887-939. DOI: 10.1080/10408398.2018.1552244. View

4.
Hollborn M, Chen R, Wiedemann P, Reichenbach A, Bringmann A, Kohen L . Cytotoxic effects of curcumin in human retinal pigment epithelial cells. PLoS One. 2013; 8(3):e59603. PMC: 3608655. DOI: 10.1371/journal.pone.0059603. View

5.
Keckeis S, Reichhart N, Roubeix C, Strauss O . Anoctamin2 (TMEM16B) forms the Ca-activated Cl channel in the retinal pigment epithelium. Exp Eye Res. 2016; 154:139-150. DOI: 10.1016/j.exer.2016.12.003. View