Unamplified and Real-Time Label-Free MiRNA-21 Detection Using Solution-Gated Graphene Transistors in Prostate Cancer Diagnosis

Overview

Journal Adv Sci (Weinh)

Specialty Biomedical Engineering

Date 2022 Dec 8

PMID 36480308

Authors

Minghua Deng

Zhanpeng Ren

Huibin Zhang

Ziqin Li

Chenglong Xue

Jianying Wang

Dan Zhang

Huan Yang

Xianbao Wang

Jinhua Li

Affiliations

Soon will be listed here.

Abstract

The incidence of prostate cancer (PCa) in men globally increases as the standard of living improves. Blood serum biomarker prostate-specific antigen (PSA) detection is the gold standard assay that do not meet the requirements of early detection. Herein, a solution-gated graphene transistor (SGGT) biosensor for the ultrasensitive and rapid quantification detection of the early prostate cancer-relevant biomarker, miRNA-21 is reported. The designed single-stranded DNA (ssDNA) probes immobilized on the Au gate can hybridize effectively with the miRNA-21 molecules targets and induce the Dirac voltage shifts of SGGT transfer curves. The limit of detection (LOD) of the sensor can reach 10 M without amplification and any chemical or biological labeling. The detection linear range is from 10 to 10 M. The sensor can realize real-time detection of the miRNA-21 molecules in less than 5 min and can well distinguish one-mismatched miRNA-21 molecule. The blood serum samples from the patients without RNA extraction and amplification are measured. The results demonstrated that the biosensor can well distinguish the cancer patients from the control group and has higher sensitivity (100%) than PSA detection (58.3%). Contrastingly, it can be found that the PSA level is not directly related to PCa.

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References

Chen Y, Gong X, Gai J . Progress and Challenges in Transfer of Large-Area Graphene Films. Adv Sci (Weinh). 2016; 3(8):1500343. PMC: 5067701. DOI: 10.1002/advs.201500343. View

Sempere L, Freemantle S, Pitha-Rowe I, Moss E, Dmitrovsky E, Ambros V . Expression profiling of mammalian microRNAs uncovers a subset of brain-expressed microRNAs with possible roles in murine and human neuronal differentiation. Genome Biol. 2004; 5(3):R13. PMC: 395763. DOI: 10.1186/gb-2004-5-3-r13. View

Yu J, Qin S, Zhang H, Wei Y, Zhu X, Yang Y . Fiber-Shaped Triboiontronic Electrochemical Transistor. Research (Wash D C). 2021; 2021:9840918. PMC: 8098052. DOI: 10.34133/2021/9840918. View

Lin P, Luo X, Hsing I, Yan F . Organic electrochemical transistors integrated in flexible microfluidic systems and used for label-free DNA sensing. Adv Mater. 2011; 23(35):4035-40. DOI: 10.1002/adma.201102017. View

Chen C, Ridzon D, Broomer A, Zhou Z, Lee D, Nguyen J . Real-time quantification of microRNAs by stem-loop RT-PCR. Nucleic Acids Res. 2005; 33(20):e179. PMC: 1292995. DOI: 10.1093/nar/gni178. View

Li S, Huang K, Fan Q, Yang S, Shen T, Mei T . Highly sensitive solution-gated graphene transistors for label-free DNA detection. Biosens Bioelectron. 2019; 136:91-96. DOI: 10.1016/j.bios.2019.04.034. View

Ghorbani F, Abbaszadeh H, Ezzati Nazhad Dolatabadi J, Aghebati-Maleki L, Yousefi M . Application of various optical and electrochemical aptasensors for detection of human prostate specific antigen: A review. Biosens Bioelectron. 2019; 142:111484. DOI: 10.1016/j.bios.2019.111484. View

Tang H, Lin P, Chan H, Yan F . Highly sensitive dopamine biosensors based on organic electrochemical transistors. Biosens Bioelectron. 2011; 26(11):4559-63. DOI: 10.1016/j.bios.2011.05.025. View

Xue T, Liang W, Li Y, Sun Y, Xiang Y, Zhang Y . Ultrasensitive detection of miRNA with an antimonene-based surface plasmon resonance sensor. Nat Commun. 2019; 10(1):28. PMC: 6318270. DOI: 10.1038/s41467-018-07947-8. View

10.

Deng M, Li J, Xiao B, Ren Z, Li Z, Yu H . Ultrasensitive Label-Free DNA Detection Based on Solution-Gated Graphene Transistors Functionalized with Carbon Quantum Dots. Anal Chem. 2022; 94(7):3320-3327. DOI: 10.1021/acs.analchem.1c05309. View

11.

Deng M, Ren Z, Zhang H, Li Z, Xue C, Wang J . Unamplified and Real-Time Label-Free miRNA-21 Detection Using Solution-Gated Graphene Transistors in Prostate Cancer Diagnosis. Adv Sci (Weinh). 2022; 10(4):e2205886. PMC: 9896035. DOI: 10.1002/advs.202205886. View

12.

Steel A, Levicky R, Herne T, Tarlov M . Immobilization of nucleic acids at solid surfaces: effect of oligonucleotide length on layer assembly. Biophys J. 2000; 79(2):975-81. PMC: 1300993. DOI: 10.1016/S0006-3495(00)76351-X. View

13.

Yang L, Liu B, Wang M, Li J, Pan W, Gao X . A Highly Sensitive Strategy for Fluorescence Imaging of MicroRNA in Living Cells and in Vivo Based on Graphene Oxide-Enhanced Signal Molecules Quenching of Molecular Beacon. ACS Appl Mater Interfaces. 2018; 10(8):6982-6990. DOI: 10.1021/acsami.7b19284. View

14.

Liss M, Petersen B, Wolf H, Prohaska E . An aptamer-based quartz crystal protein biosensor. Anal Chem. 2002; 74(17):4488-95. DOI: 10.1021/ac011294p. View

15.

Yan F, Zhang M, Li J . Solution-gated graphene transistors for chemical and biological sensors. Adv Healthc Mater. 2013; 3(3):313-31. DOI: 10.1002/adhm.201300221. View

16.

Hennelly S, Novikova I, Sanbonmatsu K . The expression platform and the aptamer: cooperativity between Mg2+ and ligand in the SAM-I riboswitch. Nucleic Acids Res. 2012; 41(3):1922-35. PMC: 3562059. DOI: 10.1093/nar/gks978. View

17.

Liu H, Yang A, Song J, Wang N, Lam P, Li Y . Ultrafast, sensitive, and portable detection of COVID-19 IgG using flexible organic electrochemical transistors. Sci Adv. 2021; 7(38):eabg8387. PMC: 8443172. DOI: 10.1126/sciadv.abg8387. View

18.

Yu H, Zhao Z, Xiao B, Deng M, Wang Z, Li Z . Aptamer-Based Solution-Gated Graphene Transistors for Highly Sensitive and Real-Time Detection of Thrombin Molecules. Anal Chem. 2021; 93(40):13673-13679. DOI: 10.1021/acs.analchem.1c03129. View

19.

Chen L, Wu J, Yan F, Ju H . Monose-modified organic electrochemical transistors for cell surface glycan analysis via competitive recognition to enzyme-labeled lectin. Mikrochim Acta. 2021; 188(8):252. DOI: 10.1007/s00604-021-04918-7. View

20.

Papadakis G, Tsortos A, Bender F, Ferapontova E, Gizeli E . Direct detection of DNA conformation in hybridization processes. Anal Chem. 2012; 84(4):1854-61. DOI: 10.1021/ac202515p. View