Gut Microbiome of Helminth-infected Indigenous Malaysians is Context Dependent

Overview

Journal Microbiome

Publisher Biomed Central

Specialties Genetics
Microbiology

Date 2022 Dec 8

PMID 36476263

Authors

Mian Zi Tee

Yi Xian Er

Alice V Easton

Nan Jiun Yap

Ii Li Lee

Joseph Devlin

Ze Chen

Kee Seong Ng

Poorani Subramanian

Angelina Angelova

Oyebola Oyesola

Shushan Sargsian

Romano Ngui

Daniel P Beiting

Christopher Chiong Meng Boey

Kek Heng Chua

Ken Cadwell

Yvonne Ai Lian Lim

Png Loke

Soo Ching Lee

Affiliations

Soon will be listed here.

Abstract

Background: While microbiomes in industrialized societies are well characterized, indigenous populations with traditional lifestyles have microbiomes that are more akin to those of ancient humans. However, metagenomic data in these populations remains scarce, and the association with soil-transmitted helminth infection status is unclear. Here, we sequenced 650 metagenomes of indigenous Malaysians from five villages with different prevalence of helminth infections.

Results: Individuals from villages with higher prevalences of helminth infections have more unmapped reads and greater microbial diversity. Microbial community diversity and composition were most strongly associated with different villages and the effects of helminth infection status on the microbiome varies by village. Longitudinal changes in the microbiome in response to albendazole anthelmintic treatment were observed in both helminth infected and uninfected individuals. Inference of bacterial population replication rates from origin of replication analysis identified specific replicating taxa associated with helminth infection.

Conclusions: Our results indicate that helminth effects on the microbiota were highly dependent on context, and effects of albendazole on the microbiota can be confounding for the interpretation of deworming studies. Furthermore, a substantial quantity of the microbiome remains unannotated, and this large dataset from an indigenous population associated with helminth infections is a valuable resource for future studies. Video Abstract.

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