Chronic Sleep Loss Disrupts Rhythmic Gene Expression in

Overview

Journal Front Physiol

Date 2022 Dec 5

PMID 36467698

Authors

Zikun Wang

Samantha Lincoln

Andrew D Nguyen

Wanhe Li

Michael W Young

Affiliations

Soon will be listed here.

Abstract

Genome-wide profiling of rhythmic gene expression has offered new avenues for studying the contribution of circadian clock to diverse biological processes. Sleep has been considered one of the most important physiological processes that are regulated by the circadian clock, however, the effects of chronic sleep loss on rhythmic gene expression remain poorly understood. In the present study, we exploited sleep mutants ( ) and ( ) as models for chronic sleep loss. We profiled the transcriptomes of head tissues collected from 4-week-old wild type flies, and at timepoints around the clock. Analysis of gene oscillation revealed a substantial loss of rhythmicity in and compared to wild type flies, with most of the affected genes common to both mutants. The disruption of gene oscillation was not due to changes in average gene expression levels. We also identified a subset of genes whose loss of rhythmicity was shared among animals with chronic sleep loss and old flies, suggesting a contribution of aging to chronic, sleep-loss-induced disruption of gene oscillation.

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