Transcriptome Analysis of Differentially Expressed CircRNAs MiRNAs and MRNAs During the Challenge of Coccidiosis

Overview

Journal Front Immunol

Specialty Allergy & Immunology

Date 2022 Dec 2

PMID 36458003

Authors

Xiaolan Chen

Zhijun Wang

Yangfeng Chen

Ibrahim Akinci

Wei Luo

Yibin Xu

Endashaw Jebessa

Damer Blake

Nick Sparks

Olivier Hanotte

Qinghua Nie

Affiliations

Soon will be listed here.

Abstract

Avian coccidiosis is a common enzootic disease caused by infection of species parasites. It causes huge economic losses in the global poultry industry. Current control using anticoccidial drugs or vaccination is limited due to drug resistance and the relatively high cost of vaccines. Improving host genetic resistance to species is considered an effective strategy for improved control of coccidiosis. Circular RNAs (circRNAs) have been found to function as biomarkers or diagnoses of various kinds of diseases. The molecular biological functions of circRNAs, miRNAs, and mRNAs related to Sasso chicken have not yet been described during species challenge. In this study, RNA-seq was used to profile the expression pattern of circRNAs, miRNAs, and mRNAs in spleens from -infected and non-infected commercial dual-purpose Sasso T445 breed chickens. Results showed a total of 40 differentially expressed circRNAs (), 31 differentially expressed miRNAs (), and 820 differentially expressed genes () between infected and non-infected chickens. Regulatory networks were constructed between differentially expressed circRNAs, miRNAs, and mRNAs to offer insights into the interaction mechanisms between chickens and spp. Functional validation of a significantly differentially expressed circRNA, , revealed that could sponge to promote the expression of the target gene () during the infection of sporozoites or LPS stimulation. Pathologically, knockdown of significantly upregulated the expression of macrophage surface markers and the macrophage activation marker, and , which indicated that might inhibit the activation of macrophage. markedly facilitated the expression of and while could attenuate the increase of and induced by , indicating that exhibited function through the -- axis. Together, our results indicate that circRNAs exhibit their resistance or susceptive roles during infection. Among these, may inhibit the activation of macrophages through the -- axis to participate in the immune response induced by infection.

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