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MiRNA-21 Promotes Differentiation of Bone Marrow Mesenchymal Stem Cells into Cardiomyocyte-like Cells by Regulating the Ajuba/Isl1 Axis Pathway

Overview
Journal Arch Med Sci
Specialty General Medicine
Date 2022 Dec 2
PMID 36457985
Authors
Wengang Yang
Lei Zhao
Song Xue
Jidong Liu
Jianggui Shan
Affiliations
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Abstract

Introduction: In this study, we aimed to investigate the role of miRNA-21 and the regulating pathway that promotes the differentiation of bone marrow mesenchymal stem cells (BMSCs).

Methods: We used miR-21-OE, miR-21-KD, ajuba-OE and ajuba-KD plasmids to infect BMSCs. The expression of miRNA-21, ajuba, Isl1 and cTnI was detected by RT-qPCR, WB and immunofluorescence staining in groups.

Results: MiRNA-21 over-expression increased the expression of Isl1, and vice versa. Ajuba over-expression decreased the expression of Isl1, and vice versa. Ajuba negatively regulated the differentiation of BMSCs into cardiomyocyte-like cells.

Conclusions: MiRNA-21 could regulate differentiation of bone marrow mesenchymal stem cells (BMSCs) to cardiomyocyte-like cells through the ajuba/Isl1 axis pathway.

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