Mesenchymal Stem Cells Were Affected by Up-regulation of MiRNA-21 in Vitro

Overview

Journal Int J Clin Exp Pathol

Specialty Pathology

Date 2020 Jan 16

PMID 31938084

Citations 2

Authors

Wengang Yang

Hui Zheng

Jianggui Dan

Chenxi Wang

Song Xue

Affiliations

Soon will be listed here.

Abstract

Background: The expression of miRNA-21 was high in cells that were derived from MSCs, but, the role of miRNA-21in the MSCs was unknown.

Material/methods: In this study, flow cytometry, which was used to identify the surface-associated antigens of MSCs. The 10 μmol/l 5-azacytidine was used to induce MSCs to differentiate to cardiomyocyte-like cells. Immunofluorescence, that was for detected the expression of troponin I (cTnI). The samples were assigned to 3 groups: the blank group, the miRNA-21 mimic group, and the negative control (NC) group. The proliferation of MSCs was detected by methyl thiazolylte-trazolium (MTT), the apoptosis of MSCs was analyzed by flow cytometry, Western-blot, which was used to identify the expression of cTNI and myoD in the MSCs.

Results: The proliferation of MSCs was increased, because of the over expression of miRNA-21; But, the apoptotic rate of the MSCs were slower in MIRNA-21 group, on account of the expression of miRNA-21 was higher than that of in the NC and CK groups. The expression of cTNI in miRNA-21 group was higher than that of in the NC and CK groups.

Conclusions: The results also suggested that, the up-regulation of miRNA-21 enhanced proliferation of MSCs, reducing the apoptosis of MSCs. MiRNA-21 promotes the differentiation of MSCs, which may pave the way for the treatment directed toward restoring miRNA-21 function for myocardial ischemia.

Citing Articles

MiRNA-21 promotes differentiation of bone marrow mesenchymal stem cells into cardiomyocyte-like cells by regulating the Ajuba/Isl1 axis pathway.

Yang W, Zhao L, Xue S, Liu J, Shan J Arch Med Sci. 2022; 18(6):1672-1677.

PMID: 36457985 PMC: 9710289. DOI: 10.5114/aoms/154956.

MicroRNA Regulation of Bone Marrow Mesenchymal Stem Cell Chondrogenesis: Toward Articular Cartilage.

Vail D, Somoza R, Caplan A Tissue Eng Part A. 2021; 28(5-6):254-269.

PMID: 34328786 PMC: 8971999. DOI: 10.1089/ten.TEA.2021.0112.

References

Park C, Moon D, Choi I, Choi B, Nam T, Rhu C . Curcumin induces apoptosis and inhibits prostaglandin E(2) production in synovial fibroblasts of patients with rheumatoid arthritis. Int J Mol Med. 2007; 20(3):365-72. View

Dull T, Zufferey R, Kelly M, Mandel R, Nguyen M, Trono D . A third-generation lentivirus vector with a conditional packaging system. J Virol. 1998; 72(11):8463-71. PMC: 110254. DOI: 10.1128/JVI.72.11.8463-8471.1998. View

Mathieu J, Ruohola-Baker H . Regulation of stem cell populations by microRNAs. Adv Exp Med Biol. 2013; 786:329-51. PMC: 3901537. DOI: 10.1007/978-94-007-6621-1_18. View

Sensebe L, Krampera M, Schrezenmeier H, Bourin P, Giordano R . Mesenchymal stem cells for clinical application. Vox Sang. 2009; 98(2):93-107. DOI: 10.1111/j.1423-0410.2009.01227.x. View

Dennis J, Charbord P . Origin and differentiation of human and murine stroma. Stem Cells. 2002; 20(3):205-14. DOI: 10.1634/stemcells.20-3-205. View

Xu W, Zhang X, Qian H, Zhu W, Sun X, Hu J . Mesenchymal stem cells from adult human bone marrow differentiate into a cardiomyocyte phenotype in vitro. Exp Biol Med (Maywood). 2004; 229(7):623-31. DOI: 10.1177/153537020422900706. View

Eguchi T, Watanabe K, Hara E, Ono M, Kuboki T, Calderwood S . OstemiR: a novel panel of microRNA biomarkers in osteoblastic and osteocytic differentiation from mesencymal stem cells. PLoS One. 2013; 8(3):e58796. PMC: 3606401. DOI: 10.1371/journal.pone.0058796. View

Lakshmipathy U, Hart R . Concise review: MicroRNA expression in multipotent mesenchymal stromal cells. Stem Cells. 2007; 26(2):356-63. PMC: 2673465. DOI: 10.1634/stemcells.2007-0625. View

Sekar D, Hairul Islam V, Thirugnanasambantham K, Saravanan S . Relevance of miR-21 in HIV and non-HIV-related lymphomas. Tumour Biol. 2014; 35(9):8387-93. DOI: 10.1007/s13277-014-2068-9. View

10.

Shin K, Lee A, Kim J, Lee S, Bae Y, Jung J . miR-21 modulates tumor outgrowth induced by human adipose tissue-derived mesenchymal stem cells in vivo. Biochem Biophys Res Commun. 2012; 422(4):633-8. DOI: 10.1016/j.bbrc.2012.05.040. View

11.

Trohatou O, Zagoura D, Bitsika V, Pappa K, Antsaklis A, Anagnou N . Sox2 suppression by miR-21 governs human mesenchymal stem cell properties. Stem Cells Transl Med. 2013; 3(1):54-68. PMC: 3902287. DOI: 10.5966/sctm.2013-0081. View

12.

Naldini L, Blomer U, Gage F, Trono D, Verma I . Efficient transfer, integration, and sustained long-term expression of the transgene in adult rat brains injected with a lentiviral vector. Proc Natl Acad Sci U S A. 1996; 93(21):11382-8. PMC: 38066. DOI: 10.1073/pnas.93.21.11382. View

13.

Fakhry M, Hamade E, Badran B, Buchet R, Magne D . Molecular mechanisms of mesenchymal stem cell differentiation towards osteoblasts. World J Stem Cells. 2013; 5(4):136-48. PMC: 3812518. DOI: 10.4252/wjsc.v5.i4.136. View

14.

FRIEDENSTEIN A, PETRAKOVA K, Kurolesova A, Frolova G . Heterotopic of bone marrow. Analysis of precursor cells for osteogenic and hematopoietic tissues. Transplantation. 1968; 6(2):230-47. View

15.

Toma C, Pittenger M, Cahill K, Byrne B, Kessler P . Human mesenchymal stem cells differentiate to a cardiomyocyte phenotype in the adult murine heart. Circulation. 2002; 105(1):93-8. DOI: 10.1161/hc0102.101442. View

16.

Wakitani S, Saito T, Caplan A . Myogenic cells derived from rat bone marrow mesenchymal stem cells exposed to 5-azacytidine. Muscle Nerve. 1995; 18(12):1417-26. DOI: 10.1002/mus.880181212. View

17.

Brighton C, HUNT R . Early histological and ultrastructural changes in medullary fracture callus. J Bone Joint Surg Am. 1991; 73(6):832-47. View

18.

Yang N, Wang G, Hu C, Shi Y, Liao L, Shi S . Tumor necrosis factor α suppresses the mesenchymal stem cell osteogenesis promoter miR-21 in estrogen deficiency-induced osteoporosis. J Bone Miner Res. 2012; 28(3):559-73. DOI: 10.1002/jbmr.1798. View

19.

Campagnoli C, Roberts I, Kumar S, Bennett P, Bellantuono I, Fisk N . Identification of mesenchymal stem/progenitor cells in human first-trimester fetal blood, liver, and bone marrow. Blood. 2001; 98(8):2396-402. DOI: 10.1182/blood.v98.8.2396. View

20.

Bakhshandeh B, Hafizi M, Ghaemi N, Soleimani M . Down-regulation of miRNA-221 triggers osteogenic differentiation in human stem cells. Biotechnol Lett. 2012; 34(8):1579-87. DOI: 10.1007/s10529-012-0934-3. View