The Fate of Early Perichondrial Cells in Developing Bones

Overview

Journal Nat Commun

Specialty Biology

Date 2022 Nov 28

PMID 36443296

Authors

Yuki Matsushita

Angel Ka Yan Chu

Chiaki Tsutsumi-Arai

Shion Orikasa

Mizuki Nagata

Sunny Y Wong

Joshua D Welch

Wanida Ono

Noriaki Ono

Affiliations

Soon will be listed here.

Abstract

In endochondral bone development, bone-forming osteoblasts and bone marrow stromal cells have dual origins in the fetal cartilage and its surrounding perichondrium. However, how early perichondrial cells distinctively contribute to developing bones remain unidentified. Here we show using in vivo cell-lineage analyses that Dlx5 fetal perichondrial cells marked by Dlx5-creER do not generate cartilage but sustainably contribute to cortical bone and marrow stromal compartments in a manner complementary to fetal chondrocyte derivatives under the regulation of Hedgehog signaling. Postnatally, Dlx5 fetal perichondrial cell derivatives preferentially populate the diaphyseal marrow stroma with a dormant adipocyte-biased state and are refractory to parathyroid hormone-induced bone anabolism. Therefore, early perichondrial cells of the fetal cartilage are destined to become an adipogenic subset of stromal cells in postnatal diaphyseal bone marrow, supporting the theory that the adult bone marrow stromal compartments are developmentally prescribed within the two distinct cells-of-origins of the fetal bone anlage.

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