Infection Modulates the Sialome and Ovariome of the Gulf Coast Tick,

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2022 Nov 28

PMID 36439862

Authors

Melina Garcia Guizzo

Khemraj Budachetri

Abdulsalam Adegoke

Jose M C Ribeiro

Shahid Karim

Affiliations

Soon will be listed here.

Abstract

The Gulf Coast tick, , is a vector of several tick-borne pathogens, including . The ability of . to persist within the tick population through transovarial and transstadial transmission, without apparently harming the ticks, contributes to the pathogen's perpetuation in the tick population. Previous studies have shown that the . load in . is regulated by the tick tissues' oxidant/antioxidant balance and the non-pathogenic tick microbiome. To obtain further insights into the interaction between tick and pathogen, we performed a bulk RNA-Seq for differential transcriptomic analysis of ovaries and salivary glands from . -infected and uninfected ticks over the feeding course on a host. The most differentially expressed functional category was of bacterial origin, exhibiting a massive overexpression of bacterial transcripts in response to the . infection. Midichloria mitochondrii and bacteria from the genus Rickettsia were mainly responsible for the overexpression of bacterial transcripts. Host genes were also modulated in . -infected tick organs. A similar number of host transcripts from all analyzed functional categories was negatively and positively modulated, revealing a global alteration of the . transcriptome in response to pathogen infection. . infection led to an increase in salivary transcripts involved in blood feeding success as well as a decrease in ovarian immune transcripts. We hypothesize that these transcriptional alterations facilitate pathogen persistence and transmission within tick population.

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