Transcriptomic and Phenotype Analysis Revealed the Role of in Stress Resistance and Virulence of Pathogenic from

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2022 Nov 28

PMID 36439857

Authors

Xiaojian Gao

Qieqi Qian

Yujie Zhu

Zhen Chen

Jingwen Xu

Wenjing Xu

Qun Jiang

Jun Wang

Xiaojun Zhang

Affiliations

Soon will be listed here.

Abstract

is widely distributed in the aquatic environment, and has been determined as a novel pathogen of various aquatic animals recently. Our previous studies have indicated caused repeated infections in , suggesting a high survival ability of the bacteria, and gene has been known to regulate stress response and virulence of many bacteria. In this study, the - RNAi strain was constructed by RNAi technology, and the regulation role of in stress resistance and virulence of was explored by transcriptomic and phenotype analysis. The transcriptome analysis showed a total of 488 differentially expressed genes (DEGs) were identified between -RNAi and wild-type strains, including 30 up-regulated genes and 458 down-regulated genes, and these down-regulated DEGs were mainly related to environmental response, biofilm formation, bacterial type II secretory system, flagellin, fimbrillin, and chemotactic protein which associated with bacterial survival and virulence. The phenotype changes also showed the - RNAi strain exhibited significantly decreasing abilities of survival in environmental stresses (starvation, salinity, low pH, and oxidative stress), biofilm production, movement, adhesion to cells, pathogenicity, and colonization to These results reveal that plays an important regulatory role in environmental stress adaptation and virulence of

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