Novel Polymorphisms of Dopa Decarboxylase Gene and Their Association with Lamb Quality Traits in Indonesian Sheep

Overview

Journal Anim Biosci

Specialty Veterinary Medicine

Date 2022 Nov 18

PMID 36397687

Authors

Ratna Sholatia Harahap

Ronny Rachman Noor

Yuni Cahya Endrawati

Huda Shalahudin Darusman

Asep Gunawan

Affiliations

Soon will be listed here.

Abstract

Objective: This study aimed to investigate the polymorphisms of the dopa decarboxylase (DDC) gene and association analysis with lamb quality and expression quantification of the DDC gene in phenotypically divergent Indonesian sheep.

Methods: The totals of 189 rams with an average body weight of 24.12 kg at 10 to 12 months were used to identify DDC gene polymorphism using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Among 189 rams, several rams representing various sheep genotypes were used for an association study between genotypes and phenotypic traits with proc general linear model (GLM) analysis. In addition, the gene expression analysis of the DDC mRNA in the phenotypically divergent sheep population was analyzed using quantitative reverse-transcription PCR.

Results: The DDC gene (g. 5377439 G>A) showed polymorphisms that indicated three genotypes: AA, AG, and GG. The DDC gene polymorphism was significantly associated (p≤0.05) with carcass characteristics including carcass percentage, carcass length, hot and cold carcass; physical properties of lamb quality including pH value; retail cut carcass; fatty acid composition such as fat content, pentadecanoic acid (C15:0), tricosylic acid (C23:0), lignoceric acid (C24:0), oleic acid (C18:1n9c), elaidic acid (C18:1n9t), nervonic acid (C24:1), linoleic acid (C18:2n6c), arachidonic acid (C20:4n6), cervonic acid (C22:6n3); and mineral content including potassium (K). The GG genotype of the DDC gene had the best association with lamb quality traits. The DDC gene expression analysis mRNA showed no significant difference (p≥0.05) between lamb quality traits.

Conclusion: The DDC gene could be used as a potential candidate gene to improve lamb quality.

Citing Articles

A comprehensive study of CYP2E1 and its role in carcass characteristics and chemical lamb meat quality in different Indonesian sheep breeds.

Harahap R, Gunawan A, Endrawati Y, Darusman H, Andersson G, Noor R PLoS One. 2024; 19(9):e0310336.

PMID: 39250496 PMC: 11383218. DOI: 10.1371/journal.pone.0310336.

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