Stimulation of Mitogenic Responses in Human Peripheral Blood Lymphocytes by Lipopolysaccharide: Serum and T Helper Cell Requirements

Overview

Journal J Immunol

Specialty Allergy & Immunology

Date 1978 Dec 1

PMID 363939

Citations 19

Authors

R A Miller

S Gartner

H S Kaplan

Affiliations

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Abstract

Stimulation of human peripheral blood lymphocytes by lipopolysaccharide (LPS) was studied by the incorporation of 3H-thymidine. Peak stimulation occurred at 7 to 9 days over a broad range of LPS concentrations. Both Escherichia coli and Salmonella typhimurium LPS were effective mitogens with S. typhimurium having slightly higher activity. There was a strict serum requirement; pooled fresh frozen human serum was found to best support stimulation. In fetal calf serum, LPS caused a reduction in culture-induced stimulation. Cell separation procedures were employed in order to study the nature of the responding cell population. It was found that only non-T cells were stimulated by LPS, but in order for maximal stimulation to occur there was a requirement for helper T cells.

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