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An Improved Protocol for Generation and Characterization of Human-induced Pluripotent Stem Cell-derived Retinal Pigment Epithelium Cells

Overview
Journal STAR Protoc
Publisher Cell Press
Specialties Biology
Biomedical Engineering
Science
Date 2022 Nov 17
PMID 36386870
Authors
Harshini Surendran
Lalitha Soundararajan
Vijay Bhaskar Reddy K
Janavi Subramani
Jonathan Stoddard
Rene Reynaga
Wayne Tschetter
Renee C Ryals
Rajarshi Pal
Affiliations
Soon will be listed here.
Abstract

We present an optimized protocol for guided differentiation of retinal pigment epithelium (RPE) cells from human-induced pluripotent stem cells (iPSC). De novo-generated RPE cells are mature, polarized, and mimic the cellular and molecular profile of primary RPE; they are also suitable for cell transplantation studies. The protocol includes an enrichment step, making it useful for large-scale GMP manufacturing. RPE cells produced following this protocol are appropriate for cell replacement therapy for macular degeneration and disease modeling. For complete details on the use and execution of this protocol, please refer to Surendran et al. (2021).

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