Identification of Lipid Regulatory Genes Modulated by Polyherbal Formulation in Chicken Liver Tissues Using Transcriptome Analysis

Overview

Journal J Adv Vet Anim Res

Specialty Veterinary Medicine

Date 2022 Nov 16

PMID 36382045

Authors

Saravanakumar Marimuthu

Subramaniyam Suresh

Prashanth DSouza

Affiliations

Soon will be listed here.

Abstract

Objective: To elucidate the cellular mechanisms of polyherbal formulation [Kolin Plus (KP)], genomics was performed to delineate the genes and pathways associated with lipid regulation through transcriptional profiling of the liver in commercial broilers raised on diets deficient in choline chloride (CCL).

Materials And Methods: The gene expression patterns were studied for four groups [normal diet: normal, choline chloride deficient (CCD), KP (400 gm/ton), and CCL (400 gm/ton)] using Agilent microarray on day 42. The hierarchical cluster analysis was carried out on 12,614 differentially expressed genes (DEGs) with a similar expression.

Results: Out of 12,614 significant DEGs, 1,926, 448, and 1,330 genes were expressed at higher rates, and 413, 482, and 1,364 were expressed at lower rates than CCD (CCD normal), CCL (CCL CCD), and KP (KP CCD), respectively. GO enrichment analysis of DEG further revealed the significant association of biological process items with the lipid, sterol, and lipoprotein metabolic processes. In particular, peroxisome proliferator-activated receptor gamma coactivator 1 alpha, carnitine palmitoyl transferase I, hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit beta, and patatin-like phospholipase domain containing 2 genes involved in fatty acid oxidation and lipase C, ABCG5, ABCG8, acetyl-CoA carboxylase, ATP citrate lyase enzyme, and peroxisome proliferator-activated receptor gamma genes involved in lipogenesis were altered by KP intervention for lipid metabolism.

Conclusions: These findings reveal that the supplementation of KP prevents fatty liver-associated problems in broiler chickens by modulating the expression of the above-mentioned genes that are responsible for the oxidation of fatty acids and lipogenesis in the liver.

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References

Kidd M . Nutritional modulation of immune function in broilers. Poult Sci. 2004; 83(4):650-7. DOI: 10.1093/ps/83.4.650. View

Jiang S, Cheng H, Cui L, Zhou Z, Hou J . Changes of blood parameters associated with bone remodeling following experimentally induced fatty liver disorder in laying hens. Poult Sci. 2013; 92(6):1443-53. DOI: 10.3382/ps.2012-02800. View

Cedo L, Santos D, Roglans N, Julve J, Pallares V, Rivas-Urbina A . Human hepatic lipase overexpression in mice induces hepatic steatosis and obesity through promoting hepatic lipogenesis and white adipose tissue lipolysis and fatty acid uptake. PLoS One. 2017; 12(12):e0189834. PMC: 5731695. DOI: 10.1371/journal.pone.0189834. View

Klein J . Membrane breakdown in acute and chronic neurodegeneration: focus on choline-containing phospholipids. J Neural Transm (Vienna). 2000; 107(8-9):1027-63. DOI: 10.1007/s007020070051. View

Vendemiale G, Grattagliano I, Caraceni P, Caraccio G, Domenicali M, DallAgata M . Mitochondrial oxidative injury and energy metabolism alteration in rat fatty liver: effect of the nutritional status. Hepatology. 2001; 33(4):808-15. DOI: 10.1053/jhep.2001.23060. View

Adams C, Reitz J, De Brabander J, Feramisco J, Li L, Brown M . Cholesterol and 25-hydroxycholesterol inhibit activation of SREBPs by different mechanisms, both involving SCAP and Insigs. J Biol Chem. 2004; 279(50):52772-80. DOI: 10.1074/jbc.M410302200. View

Guan Y, Zhang Y, Breyer M . The Role of PPARs in the Transcriptional Control of Cellular Processes. Drug News Perspect. 2003; 15(3):147-154. DOI: 10.1358/dnp.2002.15.3.840011. View

Yang Z, Zhang C, Wang J, Celi P, Ding X, Bai S . Characterization of the Intestinal Microbiota of Broiler Breeders With Different Egg Laying Rate. Front Vet Sci. 2020; 7:599337. PMC: 7732610. DOI: 10.3389/fvets.2020.599337. View

de Lima M, da Silva E, Pereira R, Romano G, de Freitas L, Dias C . Estimate of choline nutritional requirements for chicks from 1 to 21 days of age. J Anim Physiol Anim Nutr (Berl). 2018; 102(3):780-788. DOI: 10.1111/jpn.12881. View

10.

Su K, Sabeva N, Liu J, Wang Y, Bhatnagar S, van der Westhuyzen D . The ABCG5 ABCG8 sterol transporter opposes the development of fatty liver disease and loss of glycemic control independently of phytosterol accumulation. J Biol Chem. 2012; 287(34):28564-75. PMC: 3436556. DOI: 10.1074/jbc.M112.360081. View

11.

Annema W, Tietge U . Role of hepatic lipase and endothelial lipase in high-density lipoprotein-mediated reverse cholesterol transport. Curr Atheroscler Rep. 2011; 13(3):257-65. PMC: 3085744. DOI: 10.1007/s11883-011-0175-2. View

12.

Selvam R, Saravanakumar M, Suresh S, Chandrasekeran C, Prashanth D . Evaluation of polyherbal formulation and synthetic choline chloride on choline deficiency model in broilers: implications on zootechnical parameters, serum biochemistry and liver histopathology. Asian-Australas J Anim Sci. 2018; 31(11):1795-1806. PMC: 6212757. DOI: 10.5713/ajas.18.0018. View

13.

Cooke R, Silva del Rio N, Caraviello D, Bertics S, Ramos M, Grummer R . Supplemental choline for prevention and alleviation of fatty liver in dairy cattle. J Dairy Sci. 2007; 90(5):2413-8. DOI: 10.3168/jds.2006-028. View

14.

Kettunen H, Peuranen S, Tiihonen K, Saarinen M . Intestinal uptake of betaine in vitro and the distribution of methyl groups from betaine, choline, and methionine in the body of broiler chicks. Comp Biochem Physiol A Mol Integr Physiol. 2001; 128(2):269-78. DOI: 10.1016/s1095-6433(00)00301-9. View

15.

Brownsey R, Boone A, Elliott J, Kulpa J, Lee W . Regulation of acetyl-CoA carboxylase. Biochem Soc Trans. 2006; 34(Pt 2):223-7. DOI: 10.1042/BST20060223. View

16.

Musso G, Gambino R, Cassader M . Recent insights into hepatic lipid metabolism in non-alcoholic fatty liver disease (NAFLD). Prog Lipid Res. 2008; 48(1):1-26. DOI: 10.1016/j.plipres.2008.08.001. View

17.

Schadinger S, Bucher N, Schreiber B, Farmer S . PPARgamma2 regulates lipogenesis and lipid accumulation in steatotic hepatocytes. Am J Physiol Endocrinol Metab. 2005; 288(6):E1195-205. DOI: 10.1152/ajpendo.00513.2004. View

18.

Morris E, Meers G, Booth F, Fritsche K, Hardin C, Thyfault J . PGC-1α overexpression results in increased hepatic fatty acid oxidation with reduced triacylglycerol accumulation and secretion. Am J Physiol Gastrointest Liver Physiol. 2012; 303(8):G979-92. PMC: 3469696. DOI: 10.1152/ajpgi.00169.2012. View

19.

Cogburn L, Wang X, Carre W, Rejto L, Aggrey S, Duclos M . Functional genomics in chickens: development of integrated-systems microarrays for transcriptional profiling and discovery of regulatory pathways. Comp Funct Genomics. 2008; 5(3):253-61. PMC: 2447443. DOI: 10.1002/cfg.402. View

20.

Gavrilova O, Haluzik M, Matsusue K, Cutson J, Johnson L, Dietz K . Liver peroxisome proliferator-activated receptor gamma contributes to hepatic steatosis, triglyceride clearance, and regulation of body fat mass. J Biol Chem. 2003; 278(36):34268-76. DOI: 10.1074/jbc.M300043200. View