Evaluation of Polyherbal Formulation and Synthetic Choline Chloride on Choline Deficiency Model in Broilers: Implications on Zootechnical Parameters, Serum Biochemistry and Liver Histopathology

Overview

Journal Asian-Australas J Anim Sci

Specialty Veterinary Medicine

Date 2018 Apr 13

PMID 29642669

Citations 5

Authors

Ramasamy Selvam

Marimuthu Saravanakumar

Subramaniyam Suresh

C V Chandrasekeran

DSouza Prashanth

Affiliations

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Abstract

Objective: The study was designed to establish choline deficiency model (CDM) in broilers for evaluating efficacy of polyherbal formulation (PHF) in comparison with synthetic choline chloride (SCC).

Methods: A total of 2,550 one-day-old Cobb 430 broiler chicks were randomly assigned to different groups in three experiments. In experiment 1, G1 and G2 served as normal controls and were fed a basal diet with 100% soybean meal (SBM) as a major protein source supplemented with and without SCC, respectively. In G3, G4, G5, and G6 groups, SBM was replaced at 25%, 50%, 75%, and 100% by soy protein isolate (SPI) to induce a graded level of choline deficiency. In experiment 2, PHF (500 and 1,000 g/ton) in comparison with SCC (1,000 g/ton) were evaluated. In experiment 3, dose-response of PHF (200, 400, and 500 g/ton) with SCC (400 g/ton) was determined.

Results: Replacement of SBM by SPI produced a linear decrease in body weight gain (BWG) with a poor feed conversion ratio (FCR). 25% SBM replacement by SPI yielded an optimum negative impact on BWG and FCR; hence, it is considered for further studies. In experiment 2, PHF (500 and 1,000 g/ton) and SCC (1,000 g/ton) showed a similar performance in BWG, FCR and relative liver weight. In experiment 3, PHF produced an optimum efficacy at 400 g/ton and was comparable to SCC in the restoration of serum aspartate aminotransferase activity, abdominal fat, breast muscle lipid content and liver histopathological abnormalities.

Conclusion: Replacement of SBM by SPI caused choline deficiency characterised by worsening of BWG, FCR, elevation in liver enzymes and histopathological changes indicating fatty liver. CDM was found valid for evaluating SCC and PHF. It is concluded that PHF has the potential to mimic biological activities of SCC through the restoration of negative effects caused by CDM.

Citing Articles

Choline supplementation: Impact on broiler chicken performance, steatosis, and economic viability from from 1 to 42 days.

Ramalho de Lima M, Kaneko I, de Lima A, de Melo L, de Lima M, de Brito A PLoS One. 2024; 19(3):e0295488.

PMID: 38502648 PMC: 10950236. DOI: 10.1371/journal.pone.0295488.

A Polyherbal Mixture with Nutraceutical Properties for Ruminants: A Meta-Analysis and Review of BioCholine Powder.

Mendoza-Martinez G, Orzuna-Orzuna J, Roque-Jimenez J, Gloria-Trujillo A, Martinez-Garcia J, Sanchez-Lopez N Animals (Basel). 2024; 14(5).

PMID: 38473052 PMC: 11154432. DOI: 10.3390/ani14050667.

Identification of lipid regulatory genes modulated by polyherbal formulation in chicken liver tissues using transcriptome analysis.

Marimuthu S, Suresh S, DSouza P J Adv Vet Anim Res. 2022; 9(3):432-439.

PMID: 36382045 PMC: 9597915. DOI: 10.5455/javar.2022.i611.

Evaluation of polyherbal formulation in broilers fed high energy diet: Implications on zootechnical parameters, fat accretion, and serum L-carnitine levels.

DSouza P, Selvam R J Adv Vet Anim Res. 2022; 9(1):166-174.

PMID: 35445127 PMC: 8985888. DOI: 10.5455/javar.2022.i581.

Evaluation of a polyherbal formulation for the management of wet litter in broiler chickens: Implications on performance parameters, cecal moisture level, and footpad lesions.

Marimuthu S, Balasubramanian B, Selvam R, DSouza P J Adv Vet Anim Res. 2019; 6(4):536-543.

PMID: 31819883 PMC: 6882714. DOI: 10.5455/javar.2019.f379.

References

Lowry K, Baker D . Amelioration of ethionine toxicity in the chick. Poult Sci. 1987; 66(6):1028-32. DOI: 10.3382/ps.0661028. View

Zhang C, Pan M, Li B, Wang L, Mo X, Chen Y . Choline and betaine intake is inversely associated with breast cancer risk: a two-stage case-control study in China. Cancer Sci. 2012; 104(2):250-8. PMC: 7657205. DOI: 10.1111/cas.12064. View

Tranchida F, Shintu L, Rakotoniaina Z, Tchiakpe L, Deyris V, Hiol A . Metabolomic and Lipidomic Analysis of Serum Samples following Curcuma longa Extract Supplementation in High-Fructose and Saturated Fat Fed Rats. PLoS One. 2015; 10(8):e0135948. PMC: 4545834. DOI: 10.1371/journal.pone.0135948. View

Dilger R, Garrow T, Baker D . Betaine can partially spare choline in chicks but only when added to diets containing a minimal level of choline. J Nutr. 2007; 137(10):2224-8. DOI: 10.1093/jn/137.10.2224. View

Zeisel S, daCosta K, Youssef M, Hensey S . Conversion of dietary choline to trimethylamine and dimethylamine in rats: dose-response relationship. J Nutr. 1989; 119(5):800-4. DOI: 10.1093/jn/119.5.800. View

Wen Z, Tang J, Hou S, Guo Y, Huang W, Xie M . Choline requirements of White Pekin ducks from hatch to 21 days of age. Poult Sci. 2014; 93(12):3091-6. DOI: 10.3382/ps.2014-03994. View

Swain B, Johri T . Effect of supplemental methionine, choline and their combinations on the performance and immune response of broilers. Br Poult Sci. 2000; 41(1):83-8. DOI: 10.1080/00071660086457. View

Molitoris B, Baker D . Assessment of the quantity of biologically available choline in soybean meal. J Anim Sci. 1976; 42(2):481-9. DOI: 10.2527/jas1976.422481x. View

Wauben I, Wainwright P . The influence of neonatal nutrition on behavioral development: a critical appraisal. Nutr Rev. 1999; 57(2):35-44. DOI: 10.1111/j.1753-4887.1999.tb01776.x. View

10.

Asad M, Aslam M, Munir T, Nadeem A . Effect of Acacia nilotica leaves extract on hyperglycaemia, lipid profile and platelet aggregation in streptozotocin induced diabetic rats. J Ayub Med Coll Abbottabad. 2014; 23(2):3-7. View

11.

Zeisel S, daCosta K . Increase in human exposure to methylamine precursors of N-nitrosamines after eating fish. Cancer Res. 1986; 46(12 Pt 1):6136-8. View

12.

Kettunen H, Peuranen S, Tiihonen K, Saarinen M . Intestinal uptake of betaine in vitro and the distribution of methyl groups from betaine, choline, and methionine in the body of broiler chicks. Comp Biochem Physiol A Mol Integr Physiol. 2001; 128(2):269-78. DOI: 10.1016/s1095-6433(00)00301-9. View

13.

Emmert J, Baker D . A chick bioassay approach for determining the bioavailable choline concentration in normal and overheated soybean meal, canola meal and peanut meal. J Nutr. 1997; 127(5):745-52. DOI: 10.1093/jn/127.5.745. View

14.

Fanatico A, Pillai P, Emmert J, Owens C . Meat quality of slow- and fast-growing chicken genotypes fed low-nutrient or standard diets and raised indoors or with outdoor access. Poult Sci. 2007; 86(10):2245-55. DOI: 10.1093/ps/86.10.2245. View

15.

Pesti G, Benevenga N, HARPER A, Sunde M . Factors influencing the assessment of the availability of choline in feedstuffs. Poult Sci. 1981; 60(1):188-96. DOI: 10.3382/ps.0600188. View

16.

Yarru L, Settivari R, Gowda N, Antoniou E, Ledoux D, Rottinghaus G . Effects of turmeric (Curcuma longa) on the expression of hepatic genes associated with biotransformation, antioxidant, and immune systems in broiler chicks fed aflatoxin. Poult Sci. 2009; 88(12):2620-7. DOI: 10.3382/ps.2009-00204. View

17.

Harms R, Russell G . Betaine does not improve performance of laying hens when the diet contains adequate choline. Poult Sci. 2002; 81(1):99-101. DOI: 10.1093/ps/81.1.99. View

18.

Kannan N, Sakthivel K, Guruvayoorappan C . Protective Effect of Acacia nilotica (L.) against Acetaminophen-Induced Hepatocellular Damage in Wistar Rats. Adv Pharmacol Sci. 2013; 2013:987692. PMC: 3707210. DOI: 10.1155/2013/987692. View

19.

Zeisel S, Wishnok J, Blusztajn J . Formation of methylamines from ingested choline and lecithin. J Pharmacol Exp Ther. 1983; 225(2):320-4. View