CD19CD24CD38 Regulatory B Cells Deficiency Revealed Severity and Poor Prognosis in Patients with Sepsis

Overview

Journal BMC Immunol

Publisher Biomed Central

Specialty Allergy & Immunology

Date 2022 Nov 11

PMID 36357845

Authors

Chunmei Wang

Huihui Xu

Rui Gao

Fengying Leng

Fangjie Huo

Yinzhen Li

Siting Liu

Mingzheng Xu

Jianwen Bai

Affiliations

Soon will be listed here.

Abstract

Background: Sepsis still remains a major challenge in intensive care medicine with unacceptably high mortality among patients with septic shock. Due to current limitations of human CD19CD24CD38 Breg cells (Bregs) studies among sepsis, here, we tried to evaluate Bregs in severity and prognostic value in patients with sepsis.

Methods: Peripheral blood from 58 patients with sepsis and 22 healthy controls was analyzed using flow cytometry to evaluate the frequency and number of Bregs. All cases were divided into non-survived or survived group after 28 days followed up. Spearman's correlation analysis was performed on Bregs frequency and clinical indices. The area under the curve was acquired using the receiver operating characteristic analysis to assess the sensitivity and specificity of Bregs for outcome of sepsis. Survival curve analysis and binary logistic regression were applied to estimate the value of Bregs in prognosis among cases with sepsis.

Results: Sepsis patients had decreased proportions and number of Bregs. Sepsis patients with low frequency of Bregs were associated with an increased risk of septic shock. Bregs frequency is inversely associated with lactate, SOFA, and APACHE II and positively correlated with Tregs frequency. Low levels of Bregs closely correlated with septic outcomes. Numbers of Bregs were prediction factors for poor prognosis.

Conclusions: Frequency and number of Bregs decreased, and Bregs deficiency revealed poor prognosis in patients with sepsis.

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