Characterization of a Family of Scorpion Toxins Modulating Ca-Activated Cl Current in Vascular Myocytes

Overview

Journal Toxins (Basel)

Publisher MDPI

Specialty Toxicology

Date 2022 Nov 10

PMID 36356031

Authors

Jean-Luc Morel

Nathalie Mokrzycki

Guy Lippens

Herve Drobecq

Pierre Sautiere

Michel Hugues

Affiliations

Soon will be listed here.

Abstract

The pharmacology of calcium-activated chloride current is not well developed. Peptides from scorpion venom present potent pharmacological actions on ionic conductance used to characterize the function of channels but can also be helpful to develop organic pharmacological tools. Using electrophysiological recording coupled with calcium measurement, we tested the potent effect of peptides extracted from venom on the calcium-activated chloride current expressed in smooth muscle cells freshly dissociated from rat portal veins. We identified one peptide which selectively inhibited the chloride conductance without effects on either calcium signaling or calcium and potassium currents expressed in this cell type. The synthetic peptide had the same affinity, but the chemical modification of the amino acid sequence altered the efficiency to inhibit the calcium-activated chloride conductance.

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