A Method for Stabilising the XX Karyotype in Female MESC Cultures

Overview

Journal Development

Specialty Biology

Date 2022 Nov 10

PMID 36355065

Authors

Andrew Keniry

Natasha Jansz

Peter F Hickey

Kelsey A Breslin

Megan Iminitoff

Tamara Beck

Quentin Gouil

Matthew E Ritchie

Marnie E Blewitt

Affiliations

Soon will be listed here.

Abstract

Female mouse embryonic stem cells (mESCs) present differently from male mESCs in several fundamental ways; however, complications with their in vitro culture have resulted in an under-representation of female mESCs in the literature. Recent studies show that the second X chromosome in female, and more specifically the transcriptional activity from both of these chromosomes due to absent X chromosome inactivation, sets female and male mESCs apart. To avoid this undesirable state, female mESCs in culture preferentially adopt an XO karyotype, with this adaption leading to loss of their unique properties in favour of a state that is near indistinguishable from male mESCs. If female pluripotency is to be studied effectively in this system, it is crucial that high-quality cultures of XX mESCs are available. Here, we report a method for better maintaining XX female mESCs in culture that also stabilises the male karyotype and makes study of female-specific pluripotency more feasible.

Citing Articles

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