SOI-FET Sensors with Dielectrophoretic Concentration of Viruses and Proteins

Overview

Journal Biosensors (Basel)

Specialty Biotechnology

Date 2022 Nov 10

PMID 36354501

Authors

Olga Naumova

Vladimir Generalov

Dmitry Shcherbakov

Elza Zaitseva

Yuriy Zhivodkov

Anton Kozhukhov

Alexander Latyshev

Alexander Aseev

Alexander Safatov

Galina Buryak

Anastasia Cheremiskina

Julia Merkuleva

Nadezhda Rudometova

Affiliations

Soon will be listed here.

Abstract

Quick label-free virus screening and highly sensitive analytical tools/techniques are becoming extremely important in a pandemic. In this study, we developed a biosensing device based on the silicon nanoribbon multichannel and dielectrophoretic controlled sensors functionalized with SARS-CoV-2 spike antibodies for the use as a platform for the detection and studding of properties of viruses and their protein components. Replicatively defective viral particles based on vesicular stomatitis viruses and HIV-1 were used as carrier molecules to deliver the target SARS-CoV-2 spike S-proteins to sensory elements. It was shown that fully CMOS-compatible nanoribbon sensors have the subattomolar sensitivity and dynamic range of 4 orders. Specific interaction between S-proteins and antibodies leads to the accumulation of the negative charge on the sensor surface. Nonspecific interactions of the viral particles lead to the positive charge accumulation. It was shown that dielectrophoretic controlled sensors allow to estimate the effective charge of the single virus at the sensor surface and separate it from the charge associated with the binding of target proteins with the sensor surface.

Citing Articles

Investigation of Limitations in the Detection of Antibody + Antigen Complexes Using the Silicon-on-Insulator Field-Effect Transistor Biosensor.

Generalov V, Cheremiskina A, Glukhov A, Grabezhova V, Kruchinina M, Safatov A Sensors (Basel). 2023; 23(17).

PMID: 37687945 PMC: 10490761. DOI: 10.3390/s23177490.

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