Prediction of Peptides Retention Behavior in Reversed-phase Liquid Chromatography Based on Their Hydrophobicity

Overview

Journal J Sep Sci

Specialty Chemistry

Date 2022 Nov 9

PMID 36349538

Authors

Othman Al Musaimi

Oscar M Mercado Valenzo

Daryl R Williams

Affiliations

Soon will be listed here.

Abstract

Hydrophobicity is an important physicochemical property of peptides and proteins. It is responsible for their conformational changes, stability, as well as various chemical intramolecular and intermolecular interactions. Enormous efforts have been invested to study the extent of hydrophobicity and how it could influence various biological processes, in addition to its crucial role in the separation and purification endeavor as well. Here, we have reviewed various studies that were carried out to determine the hydrophobicity starting from (i) simple amino acids solubility behavior, (ii) experimental approach that was undertaken in the reversed-phase liquid chromatography mode, and ending with (iii) some examples of more advanced computational and machine learning models.

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Prediction of peptides retention behavior in reversed-phase liquid chromatography based on their hydrophobicity.

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