HAGLROS Knockdown Restrained Cell Proliferation, Migration and Invasion and Facilitated Apoptosis in Laryngeal Cancer Via MiR-138-5p/CLN5 Axis

Overview

Journal J Clin Lab Anal

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
Pathology

Date 2022 Nov 8

PMID 36347825

Authors

Dehui Fu

Yongwang Huang

Shanshan Wang

Jing Liu

Chao Li

Affiliations

Soon will be listed here.

Abstract

Background: This work investigated the role of HAGLROS in laryngeal cancer (LC).

Methods: HAGLROS expression in the head and neck squamous cell carcinoma (HNSC), target miRNAs of HAGLROS, target mRNAs of miR-138-5p, and the binding sites of HAGLROS and miR-138-5p or CLN5 and miR-138-5p were predicted through bioinformatics. HAGLROS, miR-138-5p, CLN5, Bcl-2, and Bax levels were detected by qRT-PCR and Western blot. The biological functions of LC cells were assessed through CCK-8, colony formation assays, transwell assay, and flow cytometry assay. The targeting relationship between HAGLROS and miR-138-5p or CLN5 and miR-138-5p was confirmed by dual luciferase gene reporter analysis.

Results: HAGLROS was upregulated in LC. HAGLROS-specific small interfering RNA (Si-HAGLROS) inhibited the viability, proliferation, migration, and invasion while increased the apoptosis in LC cells. MiR-138-5p was a target of HAGLROS and the miR-138-5p inhibitor reversed the effects of si-HAGLROS on LC cells. CLN5 was a target of miR-138-5p. MiR-138-5p inhibitor raised the viability, migration and invasion, and Bcl-2 expression while declined Bax expression in LC cells, with si-CLN5 performing the opposite effects and reversing the effects of miR-138-5p inhibitor.

Conclusion: Silenced HAGLROS restrained the LC cells' abilities to proliferate, migrate, and invade as well as facilitated apoptosis in LC via miR-138-5p/CLN5 axis.

Citing Articles

HAGLROS knockdown restrained cell proliferation, migration and invasion and facilitated apoptosis in laryngeal cancer via miR-138-5p/CLN5 axis.

Fu D, Huang Y, Wang S, Liu J, Li C J Clin Lab Anal. 2022; 36(12):e24712.

PMID: 36347825 PMC: 9756997. DOI: 10.1002/jcla.24712.

References

Hu Y, Guo G, Li J, Chen J, Tan P . Screening key lncRNAs with diagnostic and prognostic value for head and neck squamous cell carcinoma based on machine learning and mRNA-lncRNA co-expression network analysis. Cancer Biomark. 2019; 27(2):195-206. DOI: 10.3233/CBM-190694. View

Zhu Y, Shi L, Lei Y, Bao Y, Li Z, Ding F . Radiosensitization effect of hsa-miR-138-2-3p on human laryngeal cancer stem cells. PeerJ. 2017; 5:e3233. PMC: 5436573. DOI: 10.7717/peerj.3233. View

Wu P, Dai Z, Liu W, Zhao Z, Kong Y . Elevated long noncoding RNA HAGLROS expression correlates with clinical progression and prognosis in osteosarcoma. Eur Rev Med Pharmacol Sci. 2019; 23(4):1428-1433. DOI: 10.26355/eurrev_201902_17099. View

Li D, Feng J, Wu T, Wang Y, Sun Y, Ren J . Long intergenic noncoding RNA HOTAIR is overexpressed and regulates PTEN methylation in laryngeal squamous cell carcinoma. Am J Pathol. 2012; 182(1):64-70. DOI: 10.1016/j.ajpath.2012.08.042. View

Xing J, Li Y, Zhao H . Knockdown of CLN5 inhibits the tumorigenic properties of glioblastoma cells via the Akt/mTOR signaling pathway. Oncol Lett. 2021; 21(5):387. PMC: 7988714. DOI: 10.3892/ol.2021.12648. View

Ferlito A, Rogers S, Shaha A, Bradley P, Rinaldo A . Quality of life in head and neck cancer. Acta Otolaryngol. 2003; 123(1):5-7. DOI: 10.1080/0036554021000028072. View

Wei H, Hu J, Pu J, Tang Q, Li W, Ma R . Long noncoding RNA HAGLROS promotes cell proliferation, inhibits apoptosis and enhances autophagy via regulating miR-5095/ATG12 axis in hepatocellular carcinoma cells. Int Immunopharmacol. 2019; 73:72-80. DOI: 10.1016/j.intimp.2019.04.049. View

Li X, Yan X, Wang F, Yang Q, Luo X, Kong J . Down-regulated lncRNA SLC25A5-AS1 facilitates cell growth and inhibits apoptosis via miR-19a-3p/PTEN/PI3K/AKT signalling pathway in gastric cancer. J Cell Mol Med. 2019; 23(4):2920-2932. PMC: 6433659. DOI: 10.1111/jcmm.14200. View

Guan G, Zhang D, Wen L, Xin D, Liu Y, Yu D . Overexpression of lncRNA H19/miR-675 promotes tumorigenesis in head and neck squamous cell carcinoma. Int J Med Sci. 2016; 13(12):914-922. PMC: 5165684. DOI: 10.7150/ijms.16571. View

10.

Xia C, Dong X, Li H, Cao M, Sun D, He S . Cancer statistics in China and United States, 2022: profiles, trends, and determinants. Chin Med J (Engl). 2022; 135(5):584-590. PMC: 8920425. DOI: 10.1097/CM9.0000000000002108. View

11.

Chen J, Wu P, Xia R, Yang J, Huo X, Gu D . STAT3-induced lncRNA HAGLROS overexpression contributes to the malignant progression of gastric cancer cells via mTOR signal-mediated inhibition of autophagy. Mol Cancer. 2018; 17(1):6. PMC: 5767073. DOI: 10.1186/s12943-017-0756-y. View

12.

Zhang W, Zhang Y, Xi S . Upregulation of lncRNA HAGLROS enhances the development of nasopharyngeal carcinoma via modulating miR-100/ATG14 axis-mediated PI3K/AKT/mTOR signals. Artif Cells Nanomed Biotechnol. 2019; 47(1):3043-3052. DOI: 10.1080/21691401.2019.1640233. View

13.

Clark M, Mattick J . Long noncoding RNAs in cell biology. Semin Cell Dev Biol. 2011; 22(4):366-76. DOI: 10.1016/j.semcdb.2011.01.001. View

14.

Liz J, Esteller M . lncRNAs and microRNAs with a role in cancer development. Biochim Biophys Acta. 2015; 1859(1):169-76. DOI: 10.1016/j.bbagrm.2015.06.015. View

15.

Garavello W, Turati F, Bosetti C, Talamini R, Levi F, Lucenteforte E . Family history of cancer and the risk of laryngeal cancer: a case-control study from Italy and Switzerland. Int J Cancer. 2011; 130(3):665-70. DOI: 10.1002/ijc.26055. View

16.

Siegel R, Miller K, Fuchs H, Jemal A . Cancer statistics, 2022. CA Cancer J Clin. 2022; 72(1):7-33. DOI: 10.3322/caac.21708. View

17.

Zhang W, Liao K, Liu D . MiR-138-5p Inhibits the Proliferation of Gastric Cancer Cells by Targeting DEK. Cancer Manag Res. 2020; 12:8137-8147. PMC: 7489953. DOI: 10.2147/CMAR.S253777. View

18.

Huang H, Xiong Y, Wu Z, He Y, Gao X, Zhou Z . MIR-138-5P inhibits the progression of prostate cancer by targeting FOXC1. Mol Genet Genomic Med. 2020; 8(4):e1193. PMC: 7196466. DOI: 10.1002/mgg3.1193. View

19.

Menach P, Oburra H, Patel A . Cigarette smoking and alcohol ingestion as risk factors for laryngeal squamous cell carcinoma at kenyatta national hospital, kenya. Clin Med Insights Ear Nose Throat. 2013; 5:17-24. PMC: 3791957. DOI: 10.4137/CMENT.S8610. View

20.

Feng L, Wang R, Lian M, Ma H, He N, Liu H . Integrated Analysis of Long Noncoding RNA and mRNA Expression Profile in Advanced Laryngeal Squamous Cell Carcinoma. PLoS One. 2016; 11(12):e0169232. PMC: 5199101. DOI: 10.1371/journal.pone.0169232. View