YiaC and CobB Regulate Lysine Lactylation in Escherichia Coli

Overview

Journal Nat Commun

Specialty Biology

Date 2022 Nov 5

PMID 36333310

Authors

Hanyang Dong

Jianji Zhang

Hui Zhang

Yue Han

Congcong Lu

Chen Chen

Xiaoxia Tan

Siyu Wang

Xue Bai

Guijin Zhai

Shanshan Tian

Tao Zhang

Zhongyi Cheng

Enmin Li

Liyan Xu

Kai Zhang

Affiliations

Soon will be listed here.

Abstract

Lysine lactylation (Kla) has recently been reported to participate in regulating transcription in human cells. However, the characterization, regulatory mechanism and functional consequence of Kla in prokaryotes remain unclear. Here, we report that YiaC functions as a lysine lactylase and that CobB serves as a lysine delactylase in the regulation of metabolism. We demonstrate that YiaC catalyzes the addition of Kla, while CobB erases this PTM both in vitro and intracellularly. Moreover, we show that YdiF can catalyze the formation of a lactyl-coenzyme A, which donates lactyl group for Kla. Quantitative proteomic analysis further reveals 446 endogenous Kla sites targeted by CobB and 79 candidates targeted by YiaC in Escherichia coli (E. coli). Furthermore, we present that Kla can influence the functions of metabolic enzymes. Interestingly, we demonstrate that CobB can specifically modulate the activity of PykF by regulating K382la, promoting glycolysis and bacterial growth. Our study identifies the regulatory enzymes and functional network of Kla and reveals a Kla-mediated molecular mechanism catalyzed by CobB for glycolysis regulation in E. coli.

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