A Multi-omics Approach to Visualize Early Neuronal Differentiation from HESCs in 4D

Overview

Journal iScience

Publisher Cell Press

Date 2022 Oct 28

PMID 36304110

Authors

Athina Samara

Mari Spildrejorde

Ankush Sharma

Martin Falck

Magnus Leithaug

Stefania Modafferi

Pal Marius Bjornstad

Ganesh Acharya

Kristina Gervin

Robert Lyle

Ragnhild Eskeland

Affiliations

Soon will be listed here.

Abstract

Neuronal differentiation of pluripotent stem cells is an established method to study physiology, disease, and medication safety. However, the sequence of events in human neuronal differentiation and the ability of models to recapitulate early brain development are poorly understood. We developed a protocol optimized for the study of early human brain development and neuropharmacological applications. We comprehensively characterized gene expression and epigenetic profiles at four timepoints, because the cells differentiate from embryonic stem cells towards a heterogeneous population of progenitors, immature and mature neurons bearing telencephalic signatures. A multi-omics roadmap of neuronal differentiation, combined with searchable interactive gene analysis tools, allows for extensive exploration of early neuronal development and the effect of medications.

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A multi-omics approach to visualize early neuronal differentiation from hESCs in 4D.

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PMID: 36304110 PMC: 9593815. DOI: 10.1016/j.isci.2022.105279.

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