The Role of Inflammatory MiRNA-mRNA Interactions in PBMCs of Colorectal Cancer and Obesity Patients

Overview

Journal Immun Inflamm Dis

Specialties Allergy & Immunology
Infectious Diseases

Date 2022 Oct 27

PMID 36301024

Authors

Morteza Gholami

Marziyeh Zoughi

Bagher Larijani

Rasoul Abdollahzadeh

Reza Taslimi

Zeinab Rahmani

Alireza Kazemeini

Roobic Behboo

Farideh Razi

Milad Bastami

Shirin Hasani-Ranjbar

Mahsa M Amoli

Affiliations

Soon will be listed here.

Abstract

Introduction: Inflammation is a critical hallmark in obesity and colorectal cancer (CRC). This study aimed to investigate effective microRNA (miRNA)-messenger RNA (mRNA) interactions on inflammatory networks involved in obesity and CRC.

Methods: The literature searches were applied to identify genes expression reported on peripheral blood mononuclear cells (PBMCs) and/or blood of CRC subjects and to find inflammatory miRNA in blood samples. Furthermore, bioinformatics analysis was utilized to find inflammatory miRNA:mRNA interactions of the genes. Finally, a case-control study was set to investigate the expression of LAMC1 and GNB3 genes besides miR-10b, miR-506-3p, miR-150-5p, and miR-124-3p in CRC and control subjects.

Results: The expression of LAMC1 gene in healthy control groups was associated with body mass index (BMI) (p < .05). The level of miR-10b (p < .001), miR-506 (p < .001), and miR-124 (p <. 001) were significantly increased in PBMCs of CRC patients, while they were not associated with BMI. The level of miR-150 was associated with BMI in healthy subjects (p < .05).

Conclusions: The changes in the level of miR-506 and miR-124 in CRC patients may be associated with the regulatory role of these miRNAs on LAMC1 expression. The LAMC1 may be related to BMI, however, more observational studies on other populations are needed.

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The role of inflammatory miRNA-mRNA interactions in PBMCs of colorectal cancer and obesity patients.

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