Changes Induced by Aging and Long-Term Exercise And/or DHA Supplementation in Muscle of Obese Female Mice

Overview

Journal Nutrients

Date 2022 Oct 27

PMID 36296923

Authors

Alejandro Martinez-Gayo

Elisa Felix-Soriano

Neira Sainz

Pedro Gonzalez-Muniesa

Maria J Moreno-Aliaga

Affiliations

Soon will be listed here.

Abstract

Obesity and aging promote chronic low-grade systemic inflammation. The aim of the study was to analyze the effects of long-term physical exercise and/or omega-3 fatty acid Docosahexaenoic acid (DHA) supplementation on genes or proteins related to muscle metabolism, inflammation, muscle damage/regeneration and myokine expression in aged and obese mice. Two-month-old C57BL/6J female mice received a control or a high-fat diet for 4 months. Then, the diet-induced obese (DIO) mice were distributed into four groups: DIO, DIO + DHA, DIO + EX (treadmill training) and DIO + DHA + EX up to 18 months. Mice fed a control diet were sacrificed at 2, 6 and 18 months. Aging increased the mRNA expression of and decreased the expression of genes related to glucose uptake (, ), muscle atrophy (, , ) and myokines (, ). In aged DIO mice, exercise restored several of these changes. It increased the expression of genes related to glucose uptake (, ), fatty acid oxidation (, ), myokine expression (, ) and protein turnover, decreased expression and increased p-AKT/AKT ratio. No additional effects were observed when combining exercise and DHA. These data suggest the effectiveness of long-term training to prevent the deleterious effects of aging and obesity on muscle dysfunction.

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