Alkaloid Derivative ()-3β-Ethylamino-Pregn-17(20)-en Inhibits Triple-Negative Breast Cancer Metastasis and Angiogenesis by Targeting HSP90α

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2022 Oct 27

PMID 36296726

Authors

Xin-Yao Liu

Yu-Miao Wang

Xiang-Yu Zhang

Mei-Qi Jia

Hong-Quan Duan

Nan Qin

Ying Chen

Yang Yu

Xiao-Chuan Duan

Affiliations

Soon will be listed here.

Abstract

Metastasis is an important cause of cancer-related death. Previous studies in our laboratory found that pregnane alkaloids from had antimetastatic activity against breast cancer cells. In the current study, we demonstrated that treatment with one of the alkaloid derivatives, ()-3β-ethylamino-pregn-17(20)-en (), led to the downregulation of the HIF-1α/VEGF/VEGFR2 pathway, suppressed the phosphorylation of downstream molecules Akt, mTOR, FAK, and inhibited breast cancer metastasis and angiogenesis both in vitro and in vivo. Furthermore, the antimetastasis and antiangiogenesis effects of treatment (40 mg/kg) were more effective than that of Sorafenib (50 mg/kg). Surface plasmon resonance (SPR) analysis was performed and the result suggested that HSP90α was a direct target of . Taken together, our results suggested that compound might represent a candidate antitumor agent for metastatic breast cancer.

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