Rapid Screening of Proanthocyanidins from the Roots of Stapf and Its Preventative Effects on Dextran-Sulfate-Sodium-Induced Ulcerative Colitis

Overview

Journal Metabolites

Publisher MDPI

Date 2022 Oct 27

PMID 36295859

Authors

Mengying Lv

Yang Wang

Xiayun Wan

Bo Han

Wei Yu

Qiaoling Liang

Jie Xiang

Zheng Wang

Yanqing Liu

Yayun Qian

Fengguo Xu

Affiliations

Soon will be listed here.

Abstract

Proanthocyanidins (PACs) have been proven to exert antioxidant and anti-inflammatory effects. In this study, ultra-performance liquid chromatography (UPLC) coupled with linear ion trap-Orbitrap (LTQ-Orbitrap) high-resolution mass spectrometry was first employed to systematically screen PACs from the roots of Stapf, and its ethyl acetate extract (ERE) was found to contain PAC monomers and A-type dimeric proanthocyanidins, which were tentatively identified through characteristic fragmentation patterns. In vitro, the antioxidant activity of ERE was tested through 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2, 2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assays. In addition, ERE could inhibit the production of nitric oxide (NO) in lipopolysaccharide (LPS)-induced RAW 264.7 cells. In vivo, the preventative effects on dextran-sulfate-sodium-induced ulcerative colitis in mice was investigated. Mice were administered with ERE for 21 days, and during the last 7 days of the treatment period dextran sulfate sodium (DSS) was used to induce experimental colitis. The results showed that ERE treatment alleviated DSS-induced colitis, which was characterized by decreases in disease activity index (DAI) scores, spleen index and colon levels of TNF-α and IL-6, mitigation in pathological damage and oxidative stress and increases in colon length and IL-10 levels. In conclusion, supplementation of PACs derived from ERE may offer a new strategy for the treatment of ulcerative colitis. Moreover, our research will greatly facilitate better utilization of Ephedra plants.

Citing Articles

Decoction regulating phytochemicals' micromorphology changes and anti-inflammation activity enhancements originated from herb medicine supermolecules.

Yang L, Zhang X, Wang Z, Lin X, Zhang Y, Lu J Chin Med. 2024; 19(1):19.

PMID: 38279104 PMC: 10811931. DOI: 10.1186/s13020-023-00864-z.

Surface-Coated Acupuncture Needles as Solid-Phase Microextraction Probes for In Vivo Analysis of Bioactive Molecules in Living Plants by Mass Spectrometry.

Cheng H, Zhao X, Zhang L, Ma M, Ma X Metabolites. 2023; 13(2).

PMID: 36837839 PMC: 9968076. DOI: 10.3390/metabo13020220.

References

Huang B, Wang L, Liu M, Wu X, Lu Q, Liu R . The underlying mechanism of A-type procyanidins from peanut skin on DSS-induced ulcerative colitis mice by regulating gut microbiota and metabolism. J Food Biochem. 2022; 46(7):e14103. DOI: 10.1111/jfbc.14103. View

Kitabatake M, Matsumura Y, Ouji-Sageshima N, Nishioka T, Hara A, Kayano S . Persimmon-derived tannin ameliorates the pathogenesis of ulcerative colitis in a murine model through inhibition of the inflammatory response and alteration of microbiota. Sci Rep. 2021; 11(1):7286. PMC: 8012611. DOI: 10.1038/s41598-021-86608-1. View

Yildiz G, Yildiz Y, Ulutas P, Yaylali A, Ural M . Resveratrol Pretreatment Ameliorates TNBS Colitis in Rats. Recent Pat Endocr Metab Immune Drug Discov. 2015; 9(2):134-40. PMC: 4997944. DOI: 10.2174/1872214809666150806105737. View

Rauf A, Imran M, Abu-Izneid T, Iahtisham-Ul-Haq , Patel S, Pan X . Proanthocyanidins: A comprehensive review. Biomed Pharmacother. 2019; 116:108999. DOI: 10.1016/j.biopha.2019.108999. View

Zhang Y, Guo C, Li Y, Han X, Luo X, Chen L . Alginate Oligosaccharides Ameliorate DSS-Induced Colitis through Modulation of AMPK/NF-κB Pathway and Intestinal Microbiota. Nutrients. 2022; 14(14). PMC: 9321948. DOI: 10.3390/nu14142864. View

Tao W, Zhang Y, Shen X, Cao Y, Shi J, Ye X . Rethinking the Mechanism of the Health Benefits of Proanthocyanidins: Absorption, Metabolism, and Interaction with Gut Microbiota. Compr Rev Food Sci Food Saf. 2020; 18(4):971-985. DOI: 10.1111/1541-4337.12444. View

Zhang Y, Wang O, Ma N, Yi J, Mi H, Cai S . The preventive effect and underlying mechanism of Mill. fruits on dextran sulphate sodium-induced ulcerative colitis in mice. Food Funct. 2021; 12(20):9965-9978. DOI: 10.1039/d1fo01558c. View

Dinallo V, Di Fusco D, Di Grazia A, Laudisi F, Troncone E, Di Maggio G . The Deubiquitinating Enzyme OTUD5 Sustains Inflammatory Cytokine Response in Inflammatory Bowel Disease. J Crohns Colitis. 2021; 16(1):122-132. DOI: 10.1093/ecco-jcc/jjab121. View

Phuwapraisirisan P, Sowanthip P, Miles D, Tip-Pyang S . Reactive radical scavenging and xanthine oxidase inhibition of proanthocyanidins from Carallia brachiata. Phytother Res. 2006; 20(6):458-61. DOI: 10.1002/ptr.1877. View

10.

Monteleone G, Franze E, Troncone E, Maresca C, Marafini I . Interleukin-34 Mediates Cross-Talk Between Stromal Cells and Immune Cells in the Gut. Front Immunol. 2022; 13:873332. PMC: 9073079. DOI: 10.3389/fimmu.2022.873332. View

11.

Zhang B, Wang Z, Xin P, Wang Q, Bu H, Kuang H . Phytochemistry and pharmacology of genus Ephedra. Chin J Nat Med. 2018; 16(11):811-828. DOI: 10.1016/S1875-5364(18)30123-7. View

12.

Hagan M, Hayee B, Rodriguez-Mateos A . (Poly)phenols in Inflammatory Bowel Disease and Irritable Bowel Syndrome: A Review. Molecules. 2021; 26(7). PMC: 8036772. DOI: 10.3390/molecules26071843. View

13.

Nascimento R, da Fonseca Machado A, Galvez J, Cazarin C, Marostica Junior M . Ulcerative colitis: Gut microbiota, immunopathogenesis and application of natural products in animal models. Life Sci. 2020; 258:118129. DOI: 10.1016/j.lfs.2020.118129. View

14.

Li C, Wang L, Zhao J, Wei Y, Zhai S, Tan M . Lonicera rupicola Hook.f.et Thoms flavonoids ameliorated dysregulated inflammatory responses, intestinal barrier, and gut microbiome in ulcerative colitis via PI3K/AKT pathway. Phytomedicine. 2022; 104:154284. DOI: 10.1016/j.phymed.2022.154284. View

15.

Sheng K, Zhang G, Sun M, He S, Kong X, Wang J . Grape seed proanthocyanidin extract ameliorates dextran sulfate sodium-induced colitis through intestinal barrier improvement, oxidative stress reduction, and inflammatory cytokines and gut microbiota modulation. Food Funct. 2020; 11(9):7817-7829. DOI: 10.1039/d0fo01418d. View

16.

Calzada F, Cerda-Garcia-Rojas C, Meckes M, Cedillo-Rivera R, Bye R, Mata R . Geranins A and B, new antiprotozoal A-type proanthocyanidins from Geranium niveum. J Nat Prod. 1999; 62(5):705-9. DOI: 10.1021/np980467b. View

17.

Veloso P, Machado R, Nobre C . Mesalazine and inflammatory bowel disease - From well-established therapies to progress beyond the state of the art. Eur J Pharm Biopharm. 2021; 167:89-103. DOI: 10.1016/j.ejpb.2021.07.014. View

18.

Uddin M, Faraone I, Haque M, Rahman M, Halim M, Sonnichsen F . Insights into the leaves of Ceriscoides campanulata: Natural proanthocyanidins alleviate diabetes, inflammation, and esophageal squamous cell cancer via in vitro and in silico models. Fitoterapia. 2022; 158:105164. DOI: 10.1016/j.fitote.2022.105164. View

19.

Cai X, Han Y, Gu M, Song M, Wu X, Li Z . Dietary cranberry suppressed colonic inflammation and alleviated gut microbiota dysbiosis in dextran sodium sulfate-treated mice. Food Funct. 2019; 10(10):6331-6341. PMC: 6800821. DOI: 10.1039/c9fo01537j. View

20.

Mares J, Kumaran S, Gobbo M, Zerbe O . Interactions of lipopolysaccharide and polymyxin studied by NMR spectroscopy. J Biol Chem. 2009; 284(17):11498-506. PMC: 2670155. DOI: 10.1074/jbc.M806587200. View