Loss of Cep72 Affects the Morphology of Spermatozoa in Mice

Overview

Journal Front Physiol

Date 2022 Oct 24

PMID 36277211

Authors

Zhen Chen

Yating Xu

Dupeng Ma

Changrong Li

Ziqi Yu

Cong Liu

Tingyu Jin

Ziye Du

Zejia Li

Qi Sun

Yumin Xu

Rong Liu

Yuerong Wu

Mengcheng Luo

Affiliations

Soon will be listed here.

Abstract

The centrosome regulates mammalian meiosis by affecting recombination, synapsis, chromosome segregation, and spermiogenesis. Cep72 is one of the critical components of the centrosome. However, the physiological role of Cep72 in spermatogenesis and fertility remains unclear. In this study, we identify Cep72 as a testis-specific expression protein. Although knockout mice were viable and fertile, their sperms were morphologically abnormal with incomplete flagellum structures. Transcriptome analysis reveals significant differences in six genes (, , , , , and ), which were not previously associated with spermatogenesis. Overall, these results indicate that Cep72 participates in regulating sperm morphology and yet is dispensable for fertility in mice.

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References

Sonnen K, Schermelleh L, Leonhardt H, Nigg E . 3D-structured illumination microscopy provides novel insight into architecture of human centrosomes. Biol Open. 2012; 1(10):965-76. PMC: 3507176. DOI: 10.1242/bio.20122337. View

Kumar A, Rajendran V, Sethumadhavan R, Purohit R . CEP proteins: the knights of centrosome dynasty. Protoplasma. 2013; 250(5):965-83. DOI: 10.1007/s00709-013-0488-9. View

Gonczy P, Hatzopoulos G . Centriole assembly at a glance. J Cell Sci. 2019; 132(4). DOI: 10.1242/jcs.228833. View

Oshimori N, Li X, Ohsugi M, Yamamoto T . Cep72 regulates the localization of key centrosomal proteins and proper bipolar spindle formation. EMBO J. 2009; 28(14):2066-76. PMC: 2718283. DOI: 10.1038/emboj.2009.161. View

Chan J . A clinical overview of centrosome amplification in human cancers. Int J Biol Sci. 2011; 7(8):1122-44. PMC: 3204404. DOI: 10.7150/ijbs.7.1122. View

Lawo S, Hasegan M, Gupta G, Pelletier L . Subdiffraction imaging of centrosomes reveals higher-order organizational features of pericentriolar material. Nat Cell Biol. 2012; 14(11):1148-58. DOI: 10.1038/ncb2591. View

Han T, Wang L, Tang W, Zhang Z, Khawar M, Li G . GGNBP1 ensures proper spermiogenesis in response to stress in mice. Biochem Biophys Res Commun. 2020; 525(3):706-713. DOI: 10.1016/j.bbrc.2020.02.118. View

White S, Gharbi S, Bertani M, Chan H, Waterfield M, Timms J . Cellular responses to ErbB-2 overexpression in human mammary luminal epithelial cells: comparison of mRNA and protein expression. Br J Cancer. 2004; 90(1):173-81. PMC: 2395336. DOI: 10.1038/sj.bjc.6601458. View

Stowe T, Wilkinson C, Iqbal A, Stearns T . The centriolar satellite proteins Cep72 and Cep290 interact and are required for recruitment of BBS proteins to the cilium. Mol Biol Cell. 2012; 23(17):3322-35. PMC: 3431927. DOI: 10.1091/mbc.E12-02-0134. View

10.

Hall E, Keighren M, Ford M, Davey T, Jarman A, Smith L . Acute versus chronic loss of mammalian Azi1/Cep131 results in distinct ciliary phenotypes. PLoS Genet. 2014; 9(12):e1003928. PMC: 3887133. DOI: 10.1371/journal.pgen.1003928. View

11.

Marjanovic M, Sanchez-Huertas C, Terre B, Gomez R, Scheel J, Pacheco S . CEP63 deficiency promotes p53-dependent microcephaly and reveals a role for the centrosome in meiotic recombination. Nat Commun. 2015; 6:7676. PMC: 4499871. DOI: 10.1038/ncomms8676. View

12.

Yang H, Wang H, Jaenisch R . Generating genetically modified mice using CRISPR/Cas-mediated genome engineering. Nat Protoc. 2014; 9(8):1956-68. DOI: 10.1038/nprot.2014.134. View

13.

Nigg E, Raff J . Centrioles, centrosomes, and cilia in health and disease. Cell. 2009; 139(4):663-78. DOI: 10.1016/j.cell.2009.10.036. View

14.

Ni J, Wang J, Fu Y, Yan C, Zhu M, Jiang Y . Functional genetic variants in centrosome-related genes CEP72 and YWHAG confer susceptibility to gastric cancer. Arch Toxicol. 2020; 94(8):2861-2872. DOI: 10.1007/s00204-020-02782-7. View

15.

Shen Y, Zhang F, Li F, Jiang X, Yang Y, Li X . Loss-of-function mutations in QRICH2 cause male infertility with multiple morphological abnormalities of the sperm flagella. Nat Commun. 2019; 10(1):433. PMC: 6347614. DOI: 10.1038/s41467-018-08182-x. View

16.

Szollosi D, Calarco P, Donahue R . Absence of centrioles in the first and second meiotic spindles of mouse oocytes. J Cell Sci. 1972; 11(2):521-41. DOI: 10.1242/jcs.11.2.521. View

17.

Sha Y, Xu X, Mei L, Li P, Su Z, He X . A homozygous CEP135 mutation is associated with multiple morphological abnormalities of the sperm flagella (MMAF). Gene. 2017; 633:48-53. DOI: 10.1016/j.gene.2017.08.033. View

18.

So C, Seres K, Steyer A, Monnich E, Clift D, Pejkovska A . A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes. Science. 2019; 364(6447). PMC: 6629549. DOI: 10.1126/science.aat9557. View

19.

Jiang L, Li T, Zhang X, Zhang B, Yu C, Li Y . RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice. Curr Biol. 2017; 27(10):1498-1505.e6. DOI: 10.1016/j.cub.2017.04.017. View

20.

Li M, Huang T, Li M, Zhang C, Yu X, Yin Y . The histone modification reader ZCWPW1 is required for meiosis prophase I in male but not in female mice. Sci Adv. 2019; 5(8):eaax1101. PMC: 6693912. DOI: 10.1126/sciadv.aax1101. View