and Genes Cause Transplantation Resistance of Adipose-derived Mesenchymal Stem Cells in Type 1 Diabetes Mellitus

Overview

Journal Front Pharmacol

Date 2022 Oct 21

PMID 36267277

Authors

Michiko Horiguchi

Yuya Tsurudome

Kentaro Ushijima

Affiliations

Soon will be listed here.

Abstract

Type 1 diabetes mellitus (T1DM) is characterized by pancreatic beta cell destruction by autoantibodies and other factors, resulting in insulin secretion deficiency. Therefore, beta cell regeneration would be necessary to cure the disease. Nevertheless, the impact of type 1 diabetes on the stemness and transplantation efficiency of stem cells has not been previously described. In this study, we used next-generation sequencing to identify genes differentially expressed in T1DM adipose-derived stem cells (T1DM ADSCs) that originate from patients with type 1 diabetes. Furthermore, we evaluated their effects on transplantation efficiency following xenotransplantation into immunodeficient mice. In the T1DM ADSCs transplant group, the volume and weight of the graft were significantly reduced and the transplant efficiency was reduced. Next-generation sequencing and quantitative PCR results showed that T1DM ADSCs had significantly increased expression of and . and gene knockdown in T1DM ADSC significantly restored cell proliferation and stem cell marker expression. Therefore, transplantation of T1DM ADSCs, in which and were knocked down, into immunodeficient mice improved transplant efficiency. This study revealed that and can reduce transplantation efficiency of T1DM ADSCs. Focusing on and is expected to increase the efficiency of stem cell transplantation therapy for diabetic patients.

Citing Articles

The Diurnal Variation in Mitochondrial Gene in Human Type 2 Diabetic Mesenchymal Stem Cell Grafts.

Horiguchi M, Yoshihara K, Mizukami Y, Watanabe K, Tsurudome Y, Ushijima K Int J Mol Sci. 2025; 26(2.

PMID: 39859433 PMC: 11765740. DOI: 10.3390/ijms26020719.

Circadian Rhythms of Clock Genes After Transplantation of Mesenchymal Stem Cells with Type 2 Diabetes Mellitus.

Horiguchi M, Yoshihara K, Watanabe K, Tsurudome Y, Mizukami Y, Ushijima K Int J Mol Sci. 2024; 25(23).

PMID: 39684854 PMC: 11641841. DOI: 10.3390/ijms252313145.

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