DHA-enriched Phosphatidylcholine from Roes Regulates the Gut-liver Axis to Ameliorate High-fat Diet-induced Non-alcoholic Fatty Liver Disease

In this study, the protective effect of DHA-enriched phosphatidylcholine (DHA-PC) from roes against high-fat diet (HFD)-induced non-alcoholic fatty liver disease (NAFLD) was investigated. Our results indicated that DHA-PC significantly decreased the murine body weights, liver indexes, serum ALT, AST, and LPS levels, improved the serum lipid levels (TG, TC, LDL-C, HDL-C, NEFA), relieved the hepatic levels of the pro-inflammatory cytokines (IL-6, IL-1β, and TNF-α), and hepatic oxidative stress (MDA, SOD, GSH-Px, and CAT) in mice fed on HFD. DHA-PC significantly decreased protein expression levels of TLR4, MyD88, IKKβ, p-P65, and p-IκBα in the liver and upregulated the protein expression levels of ZO-1, occludin, and claudin-4 in the jejunum. Moreover, DHA-PC treatment alleviated intestinal dysbiosis caused by HFD. At the genus level, DHA-PC promoted the relative abundances of unclassified , NK4A136 group, , and unclassified UCG-014, while reducing the abundance of , unclassified , , , , and . Our findings suggest that DHA-PC alleviated HFD-induced NAFLD by regulating lipid metabolism and dysbiosis the gut-liver axis.