A 3-D Printed Vitrification Device Integrated with French Straws

Overview

Journal HardwareX

Date 2022 Oct 20

PMID 36263160

Authors

Nolan J Tiersch

Jacqueline Paulsen

Yue Liu

Terrence R Tiersch

Affiliations

Soon will be listed here.

Abstract

The goal of this work was to develop prototypes of open-hardware vitrification devices for sperm cryopreservation that can be integrated with existing straw platforms. The open-hardware Vitrification Device for French Straws (VD-FS) is low-cost, customizable, 3-D printable, standardized, and allows long-term sample storage and identification. The feasibility was shown for vitrifying and storing samples with multiple configurations. The results can be improved by design alternation and evaluation of various vitrification solutions. This is the first complete open-hardware vitrification device that can be integrated with existing French-straw storage systems, providing a foundation for future community-level modifications and improvements.

Citing Articles

Exploring pathways toward open-hardware ecosystems to safeguard genetic resources for biomedical research communities using aquatic model species.

Liu Y, Koch J, Arregui L, Oune A, Bodenstein S, Gutierrez-Wing M J Exp Zool B Mol Dev Evol. 2024; 342(3):278-290.

PMID: 38185943 PMC: 11099901. DOI: 10.1002/jez.b.23234.

Untangling the Gordian Knot of sea hare egg masses: An integrated open-hardware system for standardized egg strand sizing and packaging for cryopreservation research and application.

Koch J, Oune A, Bodenstein S, Tiersch T HardwareX. 2023; 16:e00476.

PMID: 38020540 PMC: 10663671. DOI: 10.1016/j.ohx.2023.e00476.

References

Liu J, Lee G, Biggers J, Toth T, Toner M . Low cryoprotectant concentration rapid vitrification of mouse oocytes and embryos. Cryobiology. 2020; 98:233-238. DOI: 10.1016/j.cryobiol.2020.10.016. View

Blackburn H, Torres L, Liu Y, Tiersch T . The Need for a Framework Addressing the Temporal Aspects of Fish Sperm Motility Leading to Community-Level Standardization. Zebrafish. 2022; 19(4):119-130. PMC: 9419943. DOI: 10.1089/zeb.2022.0006. View

Tiersch T, Monroe W . Three-dimensional printing with polylactic acid (PLA) thermoplastic offers new opportunities for cryobiology. Cryobiology. 2016; 73(3):396-398. PMC: 5116258. DOI: 10.1016/j.cryobiol.2016.10.005. View

Zuchowicz N, Belgodere J, Liu Y, Semmes I, Monroe W, Tiersch T . Low-Cost Resin 3-D Printing for Rapid Prototyping of Microdevices: Opportunities for Supporting Aquatic Germplasm Repositories. Fishes. 2023; 7(1). PMC: 9838218. DOI: 10.3390/fishes7010049. View

Liu Y, Blackburn H, Taylor S, Tiersch T . Development of germplasm repositories to assist conservation of endangered fishes: Examples from small-bodied livebearing fishes. Theriogenology. 2019; 135:138-151. PMC: 6612591. DOI: 10.1016/j.theriogenology.2019.05.020. View

Liu Y, Monroe W, Belgodere J, Choi J, Gutierrez-Wing M, Tiersch T . The emerging role of open technologies for community-based improvement of cryopreservation and quality management for repository development in aquatic species. Anim Reprod Sci. 2021; 246:106871. PMC: 9012811. DOI: 10.1016/j.anireprosci.2021.106871. View

Son W, Tan S . Comparison between slow freezing and vitrification for human embryos. Expert Rev Med Devices. 2008; 6(1):1-7. DOI: 10.1586/17434440.6.1.1. View

Childress W, Liu Y, Tiersch T . Design, alpha testing, and beta testing of a 3-D printed open-hardware portable cryopreservation device for aquatic species. J Appl Aquac. 2023; 35(1):213-236. PMC: 9909779. DOI: 10.1080/10454438.2021.1955805. View

Tao Y, Sanger E, Saewu A, Leveille M . Human sperm vitrification: the state of the art. Reprod Biol Endocrinol. 2020; 18(1):17. PMC: 7060631. DOI: 10.1186/s12958-020-00580-5. View

10.

Liu Y, Dong J, Tiersch T, Wu Q, Monroe W . An open hardware 3-D printed device for measuring tensile properties of thermoplastic filament polymers at cryogenic temperatures. Cryogenics (Guildf). 2023; 121. PMC: 9910578. DOI: 10.1016/j.cryogenics.2021.103409. View

11.

Mahmoud K, Scholkamy T, Ahmed Y, Seidel Jr G, Nawito M . Effect of different combinations of cryoprotectants on in vitro maturation of immature buffalo (Bubalus bubalis) oocytes vitrified by straw and open-pulled straw methods. Reprod Domest Anim. 2008; 45(4):565-71. DOI: 10.1111/j.1439-0531.2008.01293.x. View

12.

Sansinena M, Santos M, Zaritzky N, Chirife J . Numerical simulation of cooling rates in vitrification systems used for oocyte cryopreservation. Cryobiology. 2011; 63(1):32-7. DOI: 10.1016/j.cryobiol.2011.04.006. View

13.

Torres L, Liu Y, Guitreau A, Yang H, Tiersch T . Challenges in Development of Sperm Repositories for Biomedical Fishes: Quality Control in Small-Bodied Species. Zebrafish. 2017; 14(6):552-560. PMC: 5706620. DOI: 10.1089/zeb.2017.1426. View

14.

Vajta G, Nagy Z . Are programmable freezers still needed in the embryo laboratory? Review on vitrification. Reprod Biomed Online. 2006; 12(6):779-96. DOI: 10.1016/s1472-6483(10)61091-7. View

15.

Seki S, Mazur P . The dominance of warming rate over cooling rate in the survival of mouse oocytes subjected to a vitrification procedure. Cryobiology. 2009; 59(1):75-82. PMC: 2729265. DOI: 10.1016/j.cryobiol.2009.04.012. View

16.

Tiersch C, Liu Y, Tiersch T, Monroe W . 3-D Printed Customizable Vitrification Devices for Preservation of Genetic Resources of Aquatic Species. Aquac Eng. 2020; 90. PMC: 7434064. DOI: 10.1016/j.aquaeng.2020.102097. View

17.

Belgodere J, Liu Y, Reich E, Eades J, Tiersch T, Monroe W . Development of a Single-Piece Sperm Counting Chamber (SSCC) for Aquatic Species. Fishes. 2023; 7(5). PMC: 9838223. DOI: 10.3390/fishes7050231. View

18.

Tiersch N, Childress W, Tiersch T . Three-Dimensional Printing of Vitrification Loop Prototypes for Aquatic Species. Zebrafish. 2018; 16(3):252-261. PMC: 6531896. DOI: 10.1089/zeb.2017.1520. View

19.

Kuwayama M . Highly efficient vitrification for cryopreservation of human oocytes and embryos: the Cryotop method. Theriogenology. 2006; 67(1):73-80. DOI: 10.1016/j.theriogenology.2006.09.014. View

20.

Tiersch N, Tiersch T . Standardized Assessment of Thin-film Vitrification for Aquatic Species. N Am J Aquac. 2017; 79(4):283-288. PMC: 5724558. DOI: 10.1080/15222055.2017.1339153. View