Live Mucosal Vaccination Stimulates Potent Protection Varied CD4 and CD8 T Cell Subsets Against Wild-type 16M Challenge

Re-emerging zoonotic pathogen spp. continues to impact developing countries and persists in expanding populations of wildlife species in the US, constantly threatening infection of our domestic herds. The development of improved animal and human vaccines remains a priority. In this study, immunity to a novel live attenuated strain, termed znBM-mC, was characterized. An oral prime, intranasal (IN) boost strategy conferred exquisite protection against pulmonary challenge, with wild-type (wt) providing nearly complete protection in the lungs and spleens from brucellae colonization. Vaccination with znBM-mC showed an IFN-γ CD8 T-cell bias in the lungs as opposed to Rev 1-vaccinated mice showing IFN-γ CD4 T-cell inclination. Lung CD4 and CD8 effector memory T cells (TEMs) increased over 200-fold; and lung CD4 and CD8 resident memory T cells (TRMs) increased more than 250- and 150-fold, respectively. These T cells served as the primary producers of IFN-γ in the lungs, which was essential for vaccine clearance and the predominant cytokine generated pre-and post-challenge with wt 16M; znBM-mC growth could not be arrested in IFN-γ mice. Increases in lung TNF-α and IL-17 were also induced, with IL-17 being mostly derived from CD4 T cells. Vaccination of CD4, CD8, and B6 mice with znBM-mC conferred full protection in the lungs and spleens post-pulmonary challenge with virulent vaccination of IL-17 mice resulted in the protection of the lungs, but not the spleen. These data demonstrate the efficacy of mucosal vaccine administration for the generation of protective memory T cells against wt .