Evaluation of Free Light Chains (FLCs) Synthesis in Response to Exposure to SARS-CoV-2

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2022 Oct 14

PMID 36232891

Authors

Monika Gudowska-Sawczuk

Anna Moniuszko-Malinowska

Sara Paczek

Katarzyna Guziejko

Monika Chorazy

Barbara Mroczko

Affiliations

Soon will be listed here.

Abstract

The aim of this study is to assess the synthesis of kappa (κ) and lambda (λ) free light chains (FLCs) in the serum of patients with COVID-19. All the 120 serum samples were collected from patients with COVID-19 and from healthy controls (vaccinated and non-vaccinated against SARS-CoV-2). FLCs, IgG total, IgG4, IgG anti-Nucleocapsid (N), anti-spike S1 receptor binding domain (S-RBD) antibodies and IL-6 were measured according to the manufacturers' instructions. The concentrations of anti-N IgG, IgG total, IgG4 and IL-6 were elevated in the COVID-19 group in comparison to the vaccinated and non-vaccinated controls. The levels of anti-S-RBD IgG and κFLC were increased in COVID-19 and healthy vaccinated patients when compared to non-vaccinated controls. λFLC concentration was higher in the COVID-19 group than in the non-vaccinated group. The κ:λ ratio was lower in both COVID-19 and non-vaccinated groups in comparison to vaccinated controls. κFLC correlated with all tested parameters (anti-S-RBD IgG, anti-N IgG, λFLC, κ:λ ratio, IgG total, IgG4 and IL-6) except CRP, whereas λFLC correlated with all examined parameters except IgG4. Elevated levels of FLCs in COVID-19 and healthy vaccinated against SARS-CoV-2 patients, as well as the correlation between free light chains with specific anti-SARS-CoV-2 antibodies and IL-6, reflect hyperactivation of the immune system after contact with coronavirus. Furthermore, it seems that serum levels of FLCs might be used as predictive markers of COVID-19. Our findings suggest that free light chains are involved in SARS-CoV-2 infection. However, understanding the exact mechanism requires further investigation.

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References

Stavnezer J, Schrader C . IgH chain class switch recombination: mechanism and regulation. J Immunol. 2014; 193(11):5370-8. PMC: 4447316. DOI: 10.4049/jimmunol.1401849. View

Schiuma G, Beltrami S, Bortolotti D, Rizzo S, Rizzo R . Innate Immune Response in SARS-CoV-2 Infection. Microorganisms. 2022; 10(3). PMC: 8950297. DOI: 10.3390/microorganisms10030501. View

Della-Torre E, Lanzillotta M, Ramirez G, Dagna L, Tresoldi M . Serum IgG4 elevation in hyper-inflamed COVID-19 patients. Author's reply. Eur J Intern Med. 2021; 95:98-99. PMC: 8531494. DOI: 10.1016/j.ejim.2021.10.025. View

Yousefi Z, Taheri N, Dargahi M, Chaman R, Binesh E, Emamian M . Long-Term Persistence of Anti-SARS-COV-2 IgG Antibodies. Curr Microbiol. 2022; 79(4):96. PMC: 8853199. DOI: 10.1007/s00284-022-02800-0. View

Jing X, Xu M, Song D, Yue T, Wang Y, Zhang P . Association between inflammatory cytokines and anti-SARS-CoV-2 antibodies in hospitalized patients with COVID-19. Immun Ageing. 2022; 19(1):12. PMC: 8897556. DOI: 10.1186/s12979-022-00271-2. View

Trougakos I, Terpos E, Zirou C, Sklirou A, Apostolakou F, Gumeni S . Comparative kinetics of SARS-CoV-2 anti-spike protein RBD IgGs and neutralizing antibodies in convalescent and naïve recipients of the BNT162b2 mRNA vaccine versus COVID-19 patients. BMC Med. 2021; 19(1):208. PMC: 8380479. DOI: 10.1186/s12916-021-02090-6. View

Hampson J, Stockley R, Turner A . Free light chains: potential biomarker and predictor of mortality in alpha-1-antitrypsin deficiency and usual COPD. Respir Res. 2016; 17:34. PMC: 4815123. DOI: 10.1186/s12931-016-0348-1. View

Xia J, Zeng Y, Tan Z, Chen T, Hu W, Shuai S . Differentials of SARS-CoV-2 Viral RNA Re-positivity in Discharged COVID-19 Patients. AIDS Rev. 2021; 23(3):153-163. DOI: 10.24875/AIDSRev.21000023. View

Della-Torre E, Lanzillotta M, Strollo M, Ramirez G, Dagna L, Tresoldi M . Serum IgG4 level predicts COVID-19 related mortality. Eur J Intern Med. 2021; 93:107-109. PMC: 8461218. DOI: 10.1016/j.ejim.2021.09.012. View

10.

Nagler A, Kalaora S, Barbolin C, Gangaev A, Ketelaars S, Alon M . Identification of presented SARS-CoV-2 HLA class I and HLA class II peptides using HLA peptidomics. Cell Rep. 2021; 35(13):109305. PMC: 8185308. DOI: 10.1016/j.celrep.2021.109305. View

11.

Malecka-Gieldowska M, Folta M, Wisniewska A, Czyzewska E, Ciepiela O . Cell Population Data and Serum Polyclonal Immunoglobulin Free Light Chains in the Assessment of COVID-19 Severity. Viruses. 2021; 13(7). PMC: 8310347. DOI: 10.3390/v13071381. View

12.

Gudowska-Sawczuk M, Tarasiuk J, Kulakowska A, Kochanowicz J, Mroczko B . Kappa Free Light Chains and IgG Combined in a Novel Algorithm for the Detection of Multiple Sclerosis. Brain Sci. 2020; 10(6). PMC: 7349504. DOI: 10.3390/brainsci10060324. View

13.

Lee W, Singh G . Serum Free Light Chain Assay in Monoclonal Gammopathic Manifestations. Lab Med. 2019; 50(4):381-389. DOI: 10.1093/labmed/lmz007. View

14.

Rajkumar S, Kyle R, Therneau T, Clark R, Bradwell A, Melton 3rd L . Presence of monoclonal free light chains in the serum predicts risk of progression in monoclonal gammopathy of undetermined significance. Br J Haematol. 2004; 127(3):308-10. DOI: 10.1111/j.1365-2141.2004.05169.x. View

15.

Hepojoki S, Kareinen L, Strandin T, Vaheri A, Holthofer H, Mustonen J . Urine and Free Immunoglobulin Light Chains as Analytes for Serodiagnosis of Hantavirus Infection. Viruses. 2019; 11(9). PMC: 6783946. DOI: 10.3390/v11090809. View

16.

Ou J, Tan M, He H, Tan H, Mai J, Long Y . SARS-CoV-2 Antibodies and Associated Factors at Different Hospitalization Time Points in 192 COVID-19 Cases. J Appl Lab Med. 2021; 6(5):1133-1142. PMC: 8385817. DOI: 10.1093/jalm/jfab003. View

17.

Gudowska-Sawczuk M, Czupryna P, Moniuszko-Malinowska A, Pancewicz S, Mroczko B . Free Immunoglobulin Light Chains in Patients with Tick-Borne Encephalitis: Before and after Treatment. J Clin Med. 2021; 10(13). PMC: 8269011. DOI: 10.3390/jcm10132922. View

18.

Vazquez M, Catalan-Dibene J, Zlotnik A . B cells responses and cytokine production are regulated by their immune microenvironment. Cytokine. 2015; 74(2):318-26. PMC: 4475485. DOI: 10.1016/j.cyto.2015.02.007. View

19.

Nakano T, Matsui M, Inoue I, Awata T, Katayama S, Murakoshi T . Free immunoglobulin light chain: its biology and implications in diseases. Clin Chim Acta. 2011; 412(11-12):843-9. DOI: 10.1016/j.cca.2011.03.007. View

20.

Konen F, Schwenkenbecher P, Jendretzky K, Gingele S, Suhs K, Tumani H . The Increasing Role of Kappa Free Light Chains in the Diagnosis of Multiple Sclerosis. Cells. 2021; 10(11). PMC: 8622045. DOI: 10.3390/cells10113056. View