Baicalin Acts As an Adjuvant to Potentiate the Activity of Azithromycin Against Staphylococcus Saprophyticus Biofilm: an in Vitro, in Vivo, and Molecular Study

Overview

Journal Vet Res

Publisher Biomed Central

Specialty Veterinary Medicine

Date 2022 Oct 12

PMID 36224607

Authors

Jinli Wang

Siya Li

Jinwu Meng

Jinyue Zhu

Tianxin Qiu

Wenjia Wang

Jinxue Ding

Zhenguang Liu

Kun Li

Mujahid Lqbal

Deyun Wang

Yi Wu

Jiaguo Liu

Affiliations

Soon will be listed here.

Abstract

Staphylococcus saprophyticus is frequently involved in various difficult-to-treat infections due to the formation of biofilms. To identify useful antibiofilm strategies, this study explored the efficacy and mechanism of baicalin in enhancing the ability of azithromycin against multidrug-resistant Staphylococcus saprophyticus-Liu-2016-Liyang, China-francolin (MDRSS) biofilms in vitro and in vivo. When azithromycin was used in combination with baicalin, the minimum inhibitory concentration in biofilm (MICB) for azithromycin decreased 4- to 512-fold. Compared with the azithromycin and baicalin groups, the combination of azithromycin and baicalin could not reduce the biofilm biomass, but the dispersion rates of biofilm were decreased and the bactericidal ability was increased. Furthermore, the relative transcript levels of WalK/R system-related genes were upregulated by the addition of baicalin or azithromycin plus baicalin compared with that of the azithromycin and blank control groups. The strong correlation relationship between the WalK/R system and the bactericidal index demonstrated that baicalin enhanced the bactericidal effect of azithromycin on MDRSS biofilms by modulating the WalK/R system. In the mouse cutaneous infection model, the combination of azithromycin and baicalin succeeded in eradicating MDRSS and decreasing pathological injuries. This study indicated that baicalin has the potential to be an adjuvant to enhance the antimicrobial activity of azithromycin against MDRSS in the biofilm form by modulating the WalK/R system.

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