Epinephrine Extensively Changes the Biofilm Matrix Composition in C01 Isolated from Human Skin

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2022 Oct 7

PMID 36204611

Authors

Andrei V Gannesen

Rustam H Ziganshin

Evelina L Zdorovenko

Alena I Klimko

Elena A Ianutsevich

Olga A Danilova

Vera M Tereshina

Maxim V Gorbachevskii

Maria A Ovcharova

Ekaterina D Nevolina

Sergey V Martyanov

Alexander S Shashkov

Andrey S Dmitrenok

Andrei A Novikov

Marina V Zhurina

Ekaterina A Botchkova

Philipp V Toukach

Vladimir K Plakunov

Affiliations

Soon will be listed here.

Abstract

The importance of the impact of human hormones on commensal microbiota and microbial biofilms is established in lots of studies. In the present investigation, we continued and extended the research of epinephrine effects on the skin commensal C01 and its biofilms, and also the matrix changes during the biofilm growth. Epinephrine in concentration 4.9 × 10 M which is close to normal blood plasma level increased the amount of polysaccharides and extracellular DNA in the matrix, changed extensively its protein, lipid and polysaccharide composition. The Ef-Tu factor was one of the most abundant proteins in the matrix and its amount increased in the presence of the hormone. One of the glucose-mannose polysaccharide was absent in the matrix in presence of epinephrine after 24 h of incubation. The matrix phospholipids were also eradicated by the addition of the hormone. Hence, epinephrine has a great impact on the biofilms and their matrix composition, and this fact opens wide perspectives for the future research.

Citing Articles

Novel image-analytic approach reveals new insights in fine-tuning of slime mould network adaptation.

Rosina P, Grube M R Soc Open Sci. 2024; 11(10):240950.

PMID: 39493301 PMC: 11528663. DOI: 10.1098/rsos.240950.

Antibiotic-Polyphosphate Nanocomplexes: A Promising System for Effective Biofilm Eradication.

To D, Blanco Massani M, Coraca-Huber D, Seybold A, Ricci F, Zoller K Int J Nanomedicine. 2024; 19:9707-9725.

PMID: 39309185 PMC: 11416784. DOI: 10.2147/IJN.S473241.

Proteomic and transcriptomic analyses of biofilms and planktonic cultures in presence of epinephrine.

Gannesen A, Schelkunov M, Ziganshin R, Ovcharova M, Sukhacheva M, Makarova N AIMS Microbiol. 2024; 10(2):363-390.

PMID: 38919714 PMC: 11194618. DOI: 10.3934/microbiol.2024019.

Epinephrine Affects Ribosomes, Cell Division, and Catabolic Processes in Skin Strain C01: Revelation of the Conditionally Extensive Hormone Effect Using Orbitrap Mass Spectrometry and Proteomic Analysis.

Gannesen A, Ziganshin R, Ovcharova M, Nevolina E, Klimko A, Martyanov S Microorganisms. 2023; 11(9).

PMID: 37764026 PMC: 10535722. DOI: 10.3390/microorganisms11092181.

C-Type Natriuretic Peptide Acts as a Microorganism-Activated Regulator of the Skin Commensals and in Dual-Species Biofilms.

Ovcharova M, Schelkunov M, Geraskina O, Makarova N, Sukhacheva M, Martyanov S Biology (Basel). 2023; 12(3).

PMID: 36979128 PMC: 10045295. DOI: 10.3390/biology12030436.

References

Peterson S, Bennett L, Tornabene T . Effects of lead on the lipid composition of Micrococcus luteus cells. Appl Microbiol. 1975; 29(5):669-79. PMC: 187055. DOI: 10.1128/am.29.5.669-679.1975. View

Atrih A, Zollner P, Allmaier G, Foster S . Structural analysis of Bacillus subtilis 168 endospore peptidoglycan and its role during differentiation. J Bacteriol. 1996; 178(21):6173-83. PMC: 178487. DOI: 10.1128/jb.178.21.6173-6183.1996. View

Scardaci R, Bietto F, Racine P, Boukerb A, Lesouhaitier O, Feuilloley M . Norepinephrine and Serotonin Can Modulate the Behavior of the Probiotic NCIMB10415 towards the Host: Is a Putative Surface Sensor Involved?. Microorganisms. 2022; 10(3). PMC: 8954575. DOI: 10.3390/microorganisms10030487. View

Martyanov S, Botchkova E, Plakunov V, Gannesen A . The Impact of Norepinephrine on Mono-Species and Dual-Species Staphylococcal Biofilms. Microorganisms. 2021; 9(4). PMC: 8070549. DOI: 10.3390/microorganisms9040820. View

Vaskovsky V, Kostetsky E, Vasendin I . A universal reagent for phospholipid analysis. J Chromatogr. 1975; 114(1):129-41. DOI: 10.1016/s0021-9673(00)85249-8. View

Gannesen A, Lesouhaitier O, Racine P, Barreau M, Netrusov A, Plakunov V . Regulation of Monospecies and Mixed Biofilms Formation of Skin and by Human Natriuretic Peptides. Front Microbiol. 2019; 9:2912. PMC: 6296281. DOI: 10.3389/fmicb.2018.02912. View

Vergara E, Neira G, Gonzalez C, Cortez D, Dopson M, Holmes D . Evolution of Predicted Acid Resistance Mechanisms in the Extremely Acidophilic Genus. Genes (Basel). 2020; 11(4). PMC: 7231039. DOI: 10.3390/genes11040389. View

Clabaut M, Suet A, Racine P, Tahrioui A, Verdon J, Barreau M . Effect of 17β-estradiol on a human vaginal Lactobacillus crispatus strain. Sci Rep. 2021; 11(1):7133. PMC: 8010061. DOI: 10.1038/s41598-021-86628-x. View

Lipkind G, Shashkov A, Knirel Y, Vinogradov E, Kochetkov N . A computer-assisted structural analysis of regular polysaccharides on the basis of 13C-n.m.r. data. Carbohydr Res. 1988; 175(1):59-75. DOI: 10.1016/0008-6215(88)80156-3. View

10.

Borrel V, Thomas P, Catovic C, Racine P, Konto-Ghiorghi Y, Lefeuvre L . Acne and Stress: Impact of Catecholamines on . Front Med (Lausanne). 2019; 6:155. PMC: 6635461. DOI: 10.3389/fmed.2019.00155. View

11.

Tyanova S, Temu T, Cox J . The MaxQuant computational platform for mass spectrometry-based shotgun proteomics. Nat Protoc. 2016; 11(12):2301-2319. DOI: 10.1038/nprot.2016.136. View

12.

Ndiaye A, Mijouin L, Hillion M, Diaz S, Konto-Ghiorghi Y, Percoco G . Effect of Substance P in Staphylococcus aureus and Staphylococcus epidermidis Virulence: Implication for Skin Homeostasis. Front Microbiol. 2016; 7:506. PMC: 4832252. DOI: 10.3389/fmicb.2016.00506. View

13.

Singh S, Datta S, Narayanan K, Rajnish K . Bacterial exo-polysaccharides in biofilms: role in antimicrobial resistance and treatments. J Genet Eng Biotechnol. 2021; 19(1):140. PMC: 8460681. DOI: 10.1186/s43141-021-00242-y. View

14.

Nichols B . SEPARATION OF THE LIPIDS OF PHOTOSYNTHETIC TISSUES: IMPROVEMENTS IN ANALYSIS BY THIN-LAYER CHROMATOGRAPHY. Biochim Biophys Acta. 1963; 70:417-22. DOI: 10.1016/0006-3002(63)90771-6. View

15.

Benning C, Huang Z, Gage D . Accumulation of a novel glycolipid and a betaine lipid in cells of Rhodobacter sphaeroides grown under phosphate limitation. Arch Biochem Biophys. 1995; 317(1):103-11. DOI: 10.1006/abbi.1995.1141. View

16.

Gannesen A, Zdorovenko E, Botchkova E, Hardouin J, Massier S, Kopitsyn D . Composition of the Biofilm Matrix of Acneic Strain RT5. Front Microbiol. 2019; 10:1284. PMC: 6598116. DOI: 10.3389/fmicb.2019.01284. View

17.

Gannesen A, Schelkunov M, Geraskina O, Makarova N, Sukhacheva M, Danilova N . Epinephrine affects gene expression levels and has a complex effect on biofilm formation in strain C01 isolated from human skin. Biofilm. 2021; 3:100058. PMC: 8543384. DOI: 10.1016/j.bioflm.2021.100058. View

18.

Kapaev R, Toukach P . Simulation of 2D NMR Spectra of Carbohydrates Using GODESS Software. J Chem Inf Model. 2016; 56(6):1100-4. DOI: 10.1021/acs.jcim.6b00083. View

19.

Netzer R, Stafsnes M, Andreassen T, Goksoyr A, Bruheim P, Brautaset T . Biosynthetic pathway for γ-cyclic sarcinaxanthin in Micrococcus luteus: heterologous expression and evidence for diverse and multiple catalytic functions of C(50) carotenoid cyclases. J Bacteriol. 2010; 192(21):5688-99. PMC: 2953688. DOI: 10.1128/JB.00724-10. View

20.

Rosay T, Bazire A, Diaz S, Clamens T, Blier A, Mijouin L . Pseudomonas aeruginosa Expresses a Functional Human Natriuretic Peptide Receptor Ortholog: Involvement in Biofilm Formation. mBio. 2015; 6(4). PMC: 4550695. DOI: 10.1128/mBio.01033-15. View