Proteomic and Transcriptomic Analyses of Biofilms and Planktonic Cultures in Presence of Epinephrine

Overview

Journal AIMS Microbiol

Specialty Microbiology

Date 2024 Jun 26

PMID 38919714

Authors

A V Gannesen

M I Schelkunov

R H Ziganshin

M A Ovcharova

M V Sukhacheva

N E Makarova

S V Martyanov

N A Loginova

A M Mosolova

E V Diuvenji

E D Nevolina

V K Plakunov

Affiliations

Soon will be listed here.

Abstract

Transcriptomic and proteomic analysis were performed on 72 h biofilms of the acneic strain and planktonic cultures in the presence of epinephrine. Epinephrine predominantly downregulated genes associated with various transporter proteins. No correlation was found between proteomic and transcriptomic profiles. In control samples, the expression of 51 proteins differed between planktonic cultures and biofilms. Addition of 5 nM epinephrine reduced this number, and in the presence of 5 µM epinephrine, the difference in proteomic profiles between planktonic cultures and biofilms disappeared. According to the proteomic profiling, epinephrine itself was more effective in the case of biofilms and potentially affected the tricarboxylic acid cycle (as well as alpha-ketoglutarate decarboxylase Kgd), biotin synthesis, cell division, and transport of different compounds in cells. These findings are consistent with recent research on , suggesting that the effects of epinephrine on actinobacteria may be universal.

References

Cambronel M, Tortuel D, Biaggini K, Maillot O, Taupin L, Rehel K . Epinephrine affects motility, and increases adhesion, biofilm and virulence of Pseudomonas aeruginosa H103. Sci Rep. 2019; 9(1):20203. PMC: 6934790. DOI: 10.1038/s41598-019-56666-7. View

. UniProt: the Universal Protein Knowledgebase in 2023. Nucleic Acids Res. 2022; 51(D1):D523-D531. PMC: 9825514. DOI: 10.1093/nar/gkac1052. View

Hu T, Wei Z, Ju Q, Chen W . Sex hormones and acne: State of the art. J Dtsch Dermatol Ges. 2021; 19(4):509-515. DOI: 10.1111/ddg.14426. View

NOTELOVITZ M, Funk S, Nanavati N, Mazzeo M . Estradiol absorption from vaginal tablets in postmenopausal women. Obstet Gynecol. 2002; 99(4):556-62. DOI: 10.1016/s0029-7844(01)01385-0. View

Fourniere M, Latire T, Souak D, Feuilloley M, Bedoux G . and : Two Major Sentinels of Skin Microbiota and the Influence of Cosmetics. Microorganisms. 2020; 8(11). PMC: 7695133. DOI: 10.3390/microorganisms8111752. View

Bakker R, Ellers J, Roelofs D, Vooijs R, Dijkstra T, van Gestel C . Combining time-resolved transcriptomics and proteomics data for Adverse Outcome Pathway refinement in ecotoxicology. Sci Total Environ. 2023; 869:161740. DOI: 10.1016/j.scitotenv.2023.161740. View

Sharma V, Akavaram S, Bayles D . Genomewide transcriptional response of Escherichia coli O157:H7 to norepinephrine. BMC Genomics. 2022; 23(1):107. PMC: 8822769. DOI: 10.1186/s12864-021-08167-z. View

Jusuf N, Putra I, Sutrisno A . Correlation Between Stress Scale and Serum Substance P Level in Acne Vulgaris. Int J Gen Med. 2021; 14:681-686. PMC: 7935437. DOI: 10.2147/IJGM.S294509. View

Borrel V, Thomas P, Catovic C, Racine P, Konto-Ghiorghi Y, Lefeuvre L . Acne and Stress: Impact of Catecholamines on . Front Med (Lausanne). 2019; 6:155. PMC: 6635461. DOI: 10.3389/fmed.2019.00155. View

10.

Boyanova L . Stress hormone epinephrine (adrenaline) and norepinephrine (noradrenaline) effects on the anaerobic bacteria. Anaerobe. 2017; 44:13-19. DOI: 10.1016/j.anaerobe.2017.01.003. View

11.

Gannesen A, Ziganshin R, Ovcharova M, Nevolina E, Klimko A, Martyanov S . Epinephrine Affects Ribosomes, Cell Division, and Catabolic Processes in Skin Strain C01: Revelation of the Conditionally Extensive Hormone Effect Using Orbitrap Mass Spectrometry and Proteomic Analysis. Microorganisms. 2023; 11(9). PMC: 10535722. DOI: 10.3390/microorganisms11092181. View

12.

Reading N, Rasko D, Torres A, Sperandio V . A transcriptome study of the QseEF two-component system and the QseG membrane protein in enterohaemorrhagic Escherichia coli O157 : H7. Microbiology (Reading). 2010; 156(Pt 4):1167-1175. PMC: 2889445. DOI: 10.1099/mic.0.033027-0. View

13.

Patro R, Duggal G, Love M, Irizarry R, Kingsford C . Salmon provides fast and bias-aware quantification of transcript expression. Nat Methods. 2017; 14(4):417-419. PMC: 5600148. DOI: 10.1038/nmeth.4197. View

14.

Deutsch E, Bandeira N, Perez-Riverol Y, Sharma V, Carver J, Mendoza L . The ProteomeXchange consortium at 10 years: 2023 update. Nucleic Acids Res. 2022; 51(D1):D1539-D1548. PMC: 9825490. DOI: 10.1093/nar/gkac1040. View

15.

Li H, Durbin R . Fast and accurate short read alignment with Burrows-Wheeler transform. Bioinformatics. 2009; 25(14):1754-60. PMC: 2705234. DOI: 10.1093/bioinformatics/btp324. View

16.

Brugger S, Bomar L, Lemon K . Commensal-Pathogen Interactions along the Human Nasal Passages. PLoS Pathog. 2016; 12(7):e1005633. PMC: 4936728. DOI: 10.1371/journal.ppat.1005633. View

17.

Achermann Y, Tran B, Kang M, Harro J, Shirtliff M . Immunoproteomic Identification of In Vivo-Produced Propionibacterium acnes Proteins in a Rabbit Biofilm Infection Model. Clin Vaccine Immunol. 2015; 22(5):467-76. PMC: 4412941. DOI: 10.1128/CVI.00760-14. View

18.

Gannesen A, Schelkunov M, Geraskina O, Makarova N, Sukhacheva M, Danilova N . Epinephrine affects gene expression levels and has a complex effect on biofilm formation in strain C01 isolated from human skin. Biofilm. 2021; 3:100058. PMC: 8543384. DOI: 10.1016/j.bioflm.2021.100058. View

19.

Yosipovitch G, Tang M, Dawn A, Chen M, Goh C, Huak Y . Study of psychological stress, sebum production and acne vulgaris in adolescents. Acta Derm Venereol. 2007; 87(2):135-9. DOI: 10.2340/00015555-0231. View

20.

Niu L, Gao M, Wen S, Wang F, Shangguan H, Guo Z . Effects of Catecholamine Stress Hormones Norepinephrine and Epinephrine on Growth, Antimicrobial Susceptibility, Biofilm Formation, and Gene Expressions of Enterotoxigenic . Int J Mol Sci. 2023; 24(21). PMC: 10649963. DOI: 10.3390/ijms242115646. View