Correlation of the Position and Status of the Polar Body from the Fertilized Oocyte to the Euploid Status of Blastocysts

Overview

Journal Front Genet

Date 2022 Oct 7

PMID 36204310

Authors

Yongle Yang

Wei Tan

Changsheng Chen

Lei Jin

Bo Huang

Affiliations

Soon will be listed here.

Abstract

Polar bodies are tiny cells that are extruded during oocyte meiosis and are generally considered not essential for embryonic development. Therefore, polar bodies have been widely used as important materials for the preimplantation genetic diagnosis of human embryos. Recent studies have shown that polar bodies mediate embryonic development and that their morphology is related to embryo quality and developmental potential. However, the relationship between the emission of the polar body and embryonic euploidy remains unclear. In this study, a total of 1,360 blastocyst trophectoderm (TE) biopsies were performed, and blastocyst ploidy results were correlated with the state of polar bodies. The results showed that polar body angle size and polar body status are not directly related to whether the blastocysts are euploid, aneuploid, or mosaic ( > 0.05). Therefore, in the process of clinical embryo selection, embryologists should not predict the euploidy of blastocysts based on the state of polar bodies, thus affecting embryo selection.

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References

Huang B, Hu D, Qian K, Ai J, Li Y, Jin L . Is frozen embryo transfer cycle associated with a significantly lower incidence of ectopic pregnancy? An analysis of more than 30,000 cycles. Fertil Steril. 2014; 102(5):1345-9. DOI: 10.1016/j.fertnstert.2014.07.1245. View

Uhlmann F, Nasmyth K . Cohesion between sister chromatids must be established during DNA replication. Curr Biol. 1998; 8(20):1095-101. DOI: 10.1016/s0960-9822(98)70463-4. View

Yang D, Feng D, Gao Y, Sagnelli M, Wang X, Li D . An effective method for trophectoderm biopsy using mechanical blunt dissection: a step-by-step demonstration. Fertil Steril. 2020; 114(2):438-439. DOI: 10.1016/j.fertnstert.2020.05.035. View

Chuang T, Chang Y, Lee M, Wang H, Lai H, Chen S . The Incidence of Mosaicism for Individual Chromosome in Human Blastocysts Is Correlated With Chromosome Length. Front Genet. 2021; 11:565348. PMC: 7815765. DOI: 10.3389/fgene.2020.565348. View

Wakayama T, Yanagimachi R . Fertilisability and developmental ability of mouse oocytes with reduced amounts of cytoplasm. Zygote. 1999; 6(4):341-6. DOI: 10.1017/s096719949800029x. View

Wu L, Jin L, Chen W, Liu J, Hu J, Yu Q . The true incidence of chromosomal mosaicism after preimplantation genetic testing is much lower than that indicated by trophectoderm biopsy. Hum Reprod. 2021; 36(6):1691-1701. DOI: 10.1093/humrep/deab064. View

Briggs D, Power N, Lamb V, Rutherford A, Gosden R . Amplification of DNA sequences in polar bodies from human oocytes for diagnosis of mitochondrial disease. Lancet. 2000; 355(9214):1520-1. DOI: 10.1016/s0140-6736(00)02171-1. View

Ebner T, Yaman C, Moser M, Sommergruber M, Feichtinger O, Tews G . Prognostic value of first polar body morphology on fertilization rate and embryo quality in intracytoplasmic sperm injection. Hum Reprod. 2000; 15(2):427-30. DOI: 10.1093/humrep/15.2.427. View

Otsuki J, Nagai Y, Lopata A, Chiba K, Yasmin L, Sankai T . Symmetrical division of mouse oocytes during meiotic maturation can lead to the development of twin embryos that amalgamate to form a chimeric hermaphrodite. Hum Reprod. 2011; 27(2):380-7. DOI: 10.1093/humrep/der408. View

10.

Zong C, Lu S, Chapman A, Xie X . Genome-wide detection of single-nucleotide and copy-number variations of a single human cell. Science. 2012; 338(6114):1622-6. PMC: 3600412. DOI: 10.1126/science.1229164. View

11.

Verlinsky Y, Rechitsky S, Cieslak J, Ivakhnenko V, Wolf G, Lifchez A . Preimplantation diagnosis of single gene disorders by two-step oocyte genetic analysis using first and second polar body. Biochem Mol Med. 1998; 62(2):182-7. DOI: 10.1006/bmme.1997.2635. View

12.

Ciotti P, Notarangelo L, Morselli-Labate A, Felletti V, Porcu E, Venturoli S . First polar body morphology before ICSI is not related to embryo quality or pregnancy rate. Hum Reprod. 2004; 19(10):2334-9. DOI: 10.1093/humrep/deh433. View

13.

Revenkova E, Herrmann K, Adelfalk C, Jessberger R . Oocyte cohesin expression restricted to predictyate stages provides full fertility and prevents aneuploidy. Curr Biol. 2010; 20(17):1529-33. PMC: 2945217. DOI: 10.1016/j.cub.2010.08.024. View

14.

Ubaldi F, Rienzi L . Morphological selection of gametes. Placenta. 2008; 29 Suppl B:115-20. DOI: 10.1016/j.placenta.2008.08.009. View

15.

Gardner D, Schoolcraft W . Culture and transfer of human blastocysts. Curr Opin Obstet Gynecol. 1999; 11(3):307-11. DOI: 10.1097/00001703-199906000-00013. View

16.

Knoblich J . Asymmetric cell division during animal development. Nat Rev Mol Cell Biol. 2001; 2(1):11-20. DOI: 10.1038/35048085. View

17.

Rienzi L, Ubaldi F, Iacobelli M, Minasi M, Romano S, Ferrero S . Significance of metaphase II human oocyte morphology on ICSI outcome. Fertil Steril. 2008; 90(5):1692-700. DOI: 10.1016/j.fertnstert.2007.09.024. View

18.

Ma B, Zhang H, Jin L, Huang B . Neonatal Outcomes of Embryos Cultured in a Time-Lapse Incubation System: an Analysis of More Than 15,000 Fresh Transfer Cycles. Reprod Sci. 2021; 29(5):1524-1530. DOI: 10.1007/s43032-021-00714-z. View

19.

Verlinsky Y, Lerner S, Illkevitch N, Kuznetsov V, Kuznetsov I, Cieslak J . Is there any predictive value of first polar body morphology for embryo genotype or developmental potential?. Reprod Biomed Online. 2003; 7(3):336-41. DOI: 10.1016/s1472-6483(10)61874-3. View

20.

Huang B, Tan W, Li Z, Jin L . An artificial intelligence model (euploid prediction algorithm) can predict embryo ploidy status based on time-lapse data. Reprod Biol Endocrinol. 2021; 19(1):185. PMC: 8667440. DOI: 10.1186/s12958-021-00864-4. View