Early Weaning Leads to the Remodeling of Lipid Profile in Piglet Jejunal Crypt Cells During Post-weaning Days

Overview

Journal Anim Nutr

Date 2022 Oct 3

PMID 36189377

Authors

Yirui Shao

Xia Xiong

Kexing Wang

Pi Cheng

Lijun Zou

Jian Zhou

Ming Qi

Yulong Yin

Affiliations

Soon will be listed here.

Abstract

Reportedly, proteins involved in lipid metabolism change significantly in the jejunal crypt cells of early-weaned piglets, but the exact lipid profile change remains uncertain. In the present study, 32 piglets weaned at 21 d of age were randomly divided into 4 groups with 8 replicates. The jejunal crypt cells of a group of piglets on the post-weaning day (PWD) 1, 3, 7, and 14 were isolated per time point. Crypt cell lipid profiles were analyzed using ultra-high-performance liquid chromatography coupled with hybrid quadrupole time-of-flight mass spectrometry. This study showed that piglets suffered the greatest weaning stress on PWD 3 in terms of the lowest relative weight of the small intestine, the highest relative weight of the spleen, and the highest levels of malondialdehyde, cholesterol, and low-density lipoprotein cholesterol. The lipid profile of jejunal crypt cells including carnitine, sulfatide, sphingomyelin, hexosylceramide, and ceramide greatly changed after weaning, especially between PWD 3 and 14 ( < 0.05). The differential lipid species between these 2 d were mainly involved in the glycerophospholipid metabolism pathway. In addition, potential lipid biomarkers for weaning stress in crypt cells such as phosphatidylcholine (PC) (9:0/26:1), PC (17:0/18:2), carnitine (24:0), carnitine (22:0), sphingomyelin (d14:1/22:0), PC (P-18:0/18:4), phosphatidylethanolamine (P-16:0/20:4), phosphatidylinositol (15:1/24:4), and dihexosylceramide (d14:1/26:1) were identified. The changes in lipid profile might be related to the inflammation caused by early weaning. These findings might provide new therapeutical targets for intestinal dysfunctions caused by weaning stress.

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References

Hubler M, Kennedy A . Role of lipids in the metabolism and activation of immune cells. J Nutr Biochem. 2016; 34:1-7. PMC: 5694687. DOI: 10.1016/j.jnutbio.2015.11.002. View

Chang B, Nishikawa M, Sato E, Utsumi K, Inoue M . L-Carnitine inhibits cisplatin-induced injury of the kidney and small intestine. Arch Biochem Biophys. 2002; 405(1):55-64. DOI: 10.1016/s0003-9861(02)00342-9. View

Wang W, Zhao L, He Z, Wu N, Li Q, Qiu X . Metabolomics-based evidence of the hypoglycemic effect of Ge-Gen-Jiao-Tai-Wan in type 2 diabetic rats via UHPLC-QTOF/MS analysis. J Ethnopharmacol. 2018; 219:299-318. DOI: 10.1016/j.jep.2018.03.026. View

Chen Y, Ma Z, Shen X, Li L, Zhong J, Min L . Serum Lipidomics Profiling to Identify Biomarkers for Non-Small Cell Lung Cancer. Biomed Res Int. 2018; 2018:5276240. PMC: 6106807. DOI: 10.1155/2018/5276240. View

Espaillat M, Kew R, Obeid L . Sphingolipids in neutrophil function and inflammatory responses: Mechanisms and implications for intestinal immunity and inflammation in ulcerative colitis. Adv Biol Regul. 2016; 63:140-155. PMC: 5292058. DOI: 10.1016/j.jbior.2016.11.001. View

Bennett S, Valenzuela N, Xu H, Franko B, Fai S, Figeys D . Using neurolipidomics to identify phospholipid mediators of synaptic (dys)function in Alzheimer's Disease. Front Physiol. 2013; 4:168. PMC: 3712192. DOI: 10.3389/fphys.2013.00168. View

Barker N . Adult intestinal stem cells: critical drivers of epithelial homeostasis and regeneration. Nat Rev Mol Cell Biol. 2013; 15(1):19-33. DOI: 10.1038/nrm3721. View

Nabuurs M, Vellenga L, Wensing T, Breukink H . Weaning and the weanling diet influence the villous height and crypt depth in the small intestine of pigs and alter the concentrations of short-chain fatty acids in the large intestine and blood. J Nutr. 1998; 128(6):947-53. DOI: 10.1093/jn/128.6.947. View

Zhou Z, Zhang J, Zhang X, Mo S, Tan X, Wang L . The production of short chain fatty acid and colonic development in weaning piglets. J Anim Physiol Anim Nutr (Berl). 2019; 103(5):1530-1537. DOI: 10.1111/jpn.13164. View

10.

Campbell J, Crenshaw J, Polo J . The biological stress of early weaned piglets. J Anim Sci Biotechnol. 2013; 4(1):19. PMC: 3651348. DOI: 10.1186/2049-1891-4-19. View

11.

Castillo M, Martin-Orue S, Nofrarias M, Manzanilla E, Gasa J . Changes in caecal microbiota and mucosal morphology of weaned pigs. Vet Microbiol. 2007; 124(3-4):239-47. DOI: 10.1016/j.vetmic.2007.04.026. View

12.

Martin T . Phosphoinositide lipids as signaling molecules: common themes for signal transduction, cytoskeletal regulation, and membrane trafficking. Annu Rev Cell Dev Biol. 1999; 14:231-64. DOI: 10.1146/annurev.cellbio.14.1.231. View

13.

Lu J, Chen B, Chen T, Guo S, Xue X, Chen Q . Comprehensive metabolomics identified lipid peroxidation as a prominent feature in human plasma of patients with coronary heart diseases. Redox Biol. 2017; 12:899-907. PMC: 5415551. DOI: 10.1016/j.redox.2017.04.032. View

14.

Yang H, Fu D, Kong X, Wang W, Yang X, Nyachoti C . Dietary supplementation with N-carbamylglutamate increases the expression of intestinal amino acid transporters in weaned Huanjiang mini-pig piglets. J Anim Sci. 2013; 91(6):2740-8. DOI: 10.2527/jas.2012-5795. View

15.

Li R, Li L, Hong P, Lang W, Hui J, Yang Y . β-Carotene prevents weaning-induced intestinal inflammation by modulating gut microbiota in piglets. Anim Biosci. 2020; 34(7):1221-1234. PMC: 8255870. DOI: 10.5713/ajas.19.0499. View

16.

Palm W, Rodenfels J . Understanding the role of lipids and lipoproteins in development. Development. 2020; 147(24). DOI: 10.1242/dev.186411. View

17.

Smith F, Clark J, Overman B, Tozel C, Huang J, Rivier J . Early weaning stress impairs development of mucosal barrier function in the porcine intestine. Am J Physiol Gastrointest Liver Physiol. 2009; 298(3):G352-63. PMC: 2838512. DOI: 10.1152/ajpgi.00081.2009. View

18.

Gordon J, Hermiston M . Differentiation and self-renewal in the mouse gastrointestinal epithelium. Curr Opin Cell Biol. 1994; 6(6):795-803. DOI: 10.1016/0955-0674(94)90047-7. View

19.

Yang F, Liu M, Qin N, Li S, Yu M, Wang C . Lipidomics coupled with pathway analysis characterizes serum metabolic changes in response to potassium oxonate induced hyperuricemic rats. Lipids Health Dis. 2019; 18(1):112. PMC: 6511199. DOI: 10.1186/s12944-019-1054-z. View

20.

Suzuki T, Mochizuki K, Goda T . Localized expression of genes related to carbohydrate and lipid absorption along the crypt-villus axis of rat jejunum. Biochim Biophys Acta. 2009; 1790(12):1624-35. DOI: 10.1016/j.bbagen.2009.08.004. View