Photochemical Mechanisms of Fluorophores Employed in Single-Molecule Localization Microscopy

Overview

Journal Angew Chem Int Ed Engl

Specialty Chemistry

Date 2022 Sep 30

PMID 36177530

Authors

Kai Kikuchi

Liam D Adair

Jiarun Lin

Elizabeth J New

Amandeep Kaur

Affiliations

Soon will be listed here.

Abstract

Decoding cellular processes requires visualization of the spatial distribution and dynamic interactions of biomolecules. It is therefore not surprising that innovations in imaging technologies have facilitated advances in biomedical research. The advent of super-resolution imaging technologies has empowered biomedical researchers with the ability to answer long-standing questions about cellular processes at an entirely new level. Fluorescent probes greatly enhance the specificity and resolution of super-resolution imaging experiments. Here, we introduce key super-resolution imaging technologies, with a brief discussion on single-molecule localization microscopy (SMLM). We evaluate the chemistry and photochemical mechanisms of fluorescent probes employed in SMLM. This Review provides guidance on the identification and adoption of fluorescent probes in single molecule localization microscopy to inspire the design of next-generation fluorescent probes amenable to single-molecule imaging.

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