Solid-Phase Binding Assay for Ganglioside Binding of Human Respiroviruses

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2022 Sep 29

PMID 36175635

Authors

Takashi Suzuki

Affiliations

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Abstract

Human parainfluenza virus types 1 (hPIV-1) and 3 (hPIV-3) belong to the family Paramyxoviridae, subfamily Paramyxoviridae, and genus Respirovirus. The viruses enter by utilizing glycoproteins or glycosphingolipids (gangliosides) containing sialic acid on the cell membrane. We developed a solid-phase binding assay to evaluate hPIV-1, hPIV-3, and Sendai virus' abilities to bind to different types of gangliosides. hPIV1 and hPIV3 show strong binding to neolacto-series gangliosides containing a non-reducing terminal sialic acid residue and different specificity regarding the sialic acid linkages. This solid-phase binding assay is suitable to evaluate other orthomyxoviruses and paramyxoviruses' binding specificities utilizing sialic acids.

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