Analysis of Cell-nanoparticle Interactions and Imaging of Labeled Cells Showing Barcorded Endosomes Using Fluorescent Thiol-organosilica Nanoparticles Surface-functionalized with Polyethyleneimine

Overview

Journal Nanoscale Adv

Publisher Royal Society of Chemistry

Specialty Biotechnology

Date 2022 Sep 22

PMID 36132282

Authors

Michihiro Nakamura

Junna Nakamura

Chihiro Mochizuki

Chika Kuroda

Shigeki Kato

Tomohiro Haruta

Mayu Kakefuda

Shun Sato

Fuyuhiko Tamanoi

Norihiro Sugino

Affiliations

Soon will be listed here.

Abstract

Biomedical imaging using cell labeling is an important technique to visualize cell dynamics in the body. To label cells, thiol-organosilica nanoparticles (thiol-OS) containing fluorescein (thiol-OS/Flu) and rhodamine B (thiol-OS/Rho) were surface-functionalized with polyethyleneimine (PEI) (OS/Flu-PEI and OS/Rho-PEI) with 4 molecular weights (MWs). We hypothesized PEI structures such as brush, bent brush, bent lie-down, and coiled types on the surface depending on MWs based on dynamic light scattering and thermal gravimetric analyses. The labeling efficacy of OS/Flu-PEIs was dependent on the PEI MW and the cell type. A dual-particle administration study using thiol-OS and OS-PEIs revealed differential endosomal sorting of the particles depending on the surface of the NPs. The endosomes in the labeled cells using OS/Flu-PEI and thiol-OS/Rho revealed various patterns of fluorescence termed barcoded endosomes. The cells labeled with OS-PEI were administrated to mice intraperitoneally after labeling of peritoneal cells using thiol-OS/Rho. The labeled cells were detected and identified in cell aggregates seamlessly. The labeled cells with barcoded endosomes were also identified in cell aggregates. Biomedical imaging of OS-PEI-labeled cells combined with labeled cells showed high potential for observation of cell dynamics.

Citing Articles

Effects of Au States in Thiol-Organosilica Nanoparticles on Enzyme-like Activity for X-ray Sensitizer Application: Focus on Reactive Oxygen Species Generation in Radiotherapy.

Mochizuki C, Nakamura J, Nakamura M ACS Omega. 2023; 8(10):9569-9582.

PMID: 36936283 PMC: 10018706. DOI: 10.1021/acsomega.3c00096.

References

Urbanska A, Khanin R, Alidori S, Wong S, Mello B, Almeida B . A Genomic Profile of Local Immunity in the Melanoma Microenvironment Following Treatment with α Particle-Emitting Ultrasmall Silica Nanoparticles. Cancer Biother Radiopharm. 2020; 35(6):459-473. PMC: 7462037. DOI: 10.1089/cbr.2019.3150. View

Nakamura M, Hayashi K, Nakano M, Kanadani T, Miyamoto K, Kori T . Identification of polyethylene glycol-resistant macrophages on stealth imaging in vitro using fluorescent organosilica nanoparticles. ACS Nano. 2015; 9(2):1058-71. DOI: 10.1021/nn502319r. View

Hendrikx P, Martens C, Hagenbeek A, Keij J, Visser J . Homing of fluorescently labeled murine hematopoietic stem cells. Exp Hematol. 1996; 24(2):129-40. View

Nakamura M, Ishimura K . One-pot synthesis and characterization of three kinds of thiol-organosilica nanoparticles. Langmuir. 2008; 24(9):5099-108. DOI: 10.1021/la703395w. View

Haladjova E, Rangelov S, Tsvetanov C . Thermoresponsive Polyoxazolines as Vectors for Transfection of Nucleic Acids. Polymers (Basel). 2020; 12(11). PMC: 7694630. DOI: 10.3390/polym12112609. View

Chandrasekaran R, Madheswaran T, Tharmalingam N, Bose R, Park H, Ha D . Labeling and tracking cells with gold nanoparticles. Drug Discov Today. 2020; 26(1):94-105. DOI: 10.1016/j.drudis.2020.10.020. View

Zanganeh S, Hutter G, Spitler R, Lenkov O, Mahmoudi M, Shaw A . Iron oxide nanoparticles inhibit tumour growth by inducing pro-inflammatory macrophage polarization in tumour tissues. Nat Nanotechnol. 2016; 11(11):986-994. PMC: 5198777. DOI: 10.1038/nnano.2016.168. View

Bernsen M, Guenoun J, van Tiel S, Krestin G . Nanoparticles and clinically applicable cell tracking. Br J Radiol. 2015; 88(1054):20150375. PMC: 4730978. DOI: 10.1259/bjr.20150375. View

Brown E, Verkade P . The use of markers for correlative light electron microscopy. Protoplasma. 2010; 244(1-4):91-7. DOI: 10.1007/s00709-010-0165-1. View

10.

Xia T, Kovochich M, Liong M, Meng H, Kabehie S, George S . Polyethyleneimine coating enhances the cellular uptake of mesoporous silica nanoparticles and allows safe delivery of siRNA and DNA constructs. ACS Nano. 2009; 3(10):3273-86. PMC: 3900639. DOI: 10.1021/nn900918w. View

11.

Feng Q, Liu Y, Huang J, Chen K, Huang J, Xiao K . Uptake, distribution, clearance, and toxicity of iron oxide nanoparticles with different sizes and coatings. Sci Rep. 2018; 8(1):2082. PMC: 5794763. DOI: 10.1038/s41598-018-19628-z. View

12.

Barata-Antunes C, Alves R, Talaia G, Casal M, Geros H, Mans R . Endocytosis of nutrient transporters in fungi: The ART of connecting signaling and trafficking. Comput Struct Biotechnol J. 2021; 19:1713-1737. PMC: 8050425. DOI: 10.1016/j.csbj.2021.03.013. View

13.

Hobel S, Aigner A . Polyethylenimines for siRNA and miRNA delivery in vivo. Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2013; 5(5):484-501. DOI: 10.1002/wnan.1228. View

14.

Munz C . Autophagy proteins influence endocytosis for MHC restricted antigen presentation. Semin Cancer Biol. 2019; 66:110-115. DOI: 10.1016/j.semcancer.2019.03.005. View

15.

Shimomura O, Johnson F, Saiga Y . Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea. J Cell Comp Physiol. 1962; 59:223-39. DOI: 10.1002/jcp.1030590302. View

16.

Ahrens E, Bulte J . Tracking immune cells in vivo using magnetic resonance imaging. Nat Rev Immunol. 2013; 13(10):755-63. PMC: 3886235. DOI: 10.1038/nri3531. View

17.

Piao M, Kang K, Lee I, Kim H, Kim S, Choi J . Silver nanoparticles induce oxidative cell damage in human liver cells through inhibition of reduced glutathione and induction of mitochondria-involved apoptosis. Toxicol Lett. 2010; 201(1):92-100. DOI: 10.1016/j.toxlet.2010.12.010. View

18.

Dadhich R, Kapoor S . Various Facets of Pathogenic Lipids in Infectious Diseases: Exploring Virulent Lipid-Host Interactome and Their Druggability. J Membr Biol. 2020; 253(5):399-423. PMC: 7443855. DOI: 10.1007/s00232-020-00135-0. View

19.

Xia T, Kovochich M, Liong M, Zink J, Nel A . Cationic polystyrene nanosphere toxicity depends on cell-specific endocytic and mitochondrial injury pathways. ACS Nano. 2009; 2(1):85-96. DOI: 10.1021/nn700256c. View

20.

Kang H, Zhang K, Hong Wong D, Han F, Li B, Bian L . Near-infrared light-controlled regulation of intracellular calcium to modulate macrophage polarization. Biomaterials. 2018; 178:681-696. DOI: 10.1016/j.biomaterials.2018.03.007. View